We narrowed to 8,188 results for: pcas

1. pCAG-EGxxFP-Cetn1

   Plasmid

   #50717

   Purpose

   Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Insert

   Cetn1 (Cetn1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MH6-RspCas13d

   Plasmid

   #108305

   Purpose

   Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Insert

   RspCas13d
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9(1.1)-Puro

   Plasmid

   #117686

   Purpose

   The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

   Depositor

   Luc Leyns

   Article

   Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR

   Tags

   PuroR (R166H)

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   U6

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAX Itgb1-FLAG

   Plasmid

   #30153

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   integrin beta 1 (Itgb1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCAGGS-FLAG-mISG15

   Plasmid

   #12443

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   ISG15
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Myr-p110-IH

   Plasmid

   #15689

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

   Insert

   p110 alpha (Pik3ca Mouse)
   

   Tags

   Myr

   Expression

   Mammalian

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

7. Tubp-Gal80 in pCaSpeR4

   Plasmid

   #17748

   Depositor

   Liqun Luo

   Article

   Lee et al Neuron. 1999 Mar . 22(3):451-61.

   Insert

   GAL80 (GAL80 Budding Yeast)
   

   Tags

   SV40 polyA and Tubulin 1alpha promoter

   Expression

   Insect

   Available Since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG mito-mTagBFP2

   Plasmid

   #105011

   Purpose

   Mitochondrial marker

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-PIF3-mEGFP

   Plasmid

   #100283

   Purpose

   Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome interacting factor 3 (PIF3 Mustard Weed)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N-terminus (1-100 aa) of PIF3 is used

   Promoter

   CAG promoter

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-SpCas9-HF1

    Plasmid

    #108301

    Purpose

    pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-eNpHR3.0-mRuby2-ST

    Plasmid

    #109134

    Purpose

    Halorhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eNpHR3.0-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-5HA-mISG15

    Plasmid

    #12444

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    ISG15
    

    Tags

    5xHA

    Expression

    Mammalian

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pCAGGS-ChrimsonR-mRuby2-ST

    Plasmid

    #105447

    Purpose

    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Insert

    ChrimsonR-mRuby2-ST
    

    Tags

    mRuby2 and soma targeting motif

    Expression

    Mammalian

    Mutation

    K176R in Chrimson

    Promoter

    pCAGGS

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-ChroME-mRuby2-ST

    Plasmid

    #108902

    Purpose

    Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG/hArf4(WT)-mCherry

    Plasmid

    #79406

    Purpose

    Expresses C-terminally mCherry-tagged Arf4(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pCAG-Scarlet-Kif5a

    Plasmid

    #118745

    Purpose

    Expresses scarlet tagged Kif5a

    Depositor

    Beverly Koller

    Insert

    Kif5a cDNA (Kif5a Mouse)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAP03-sp44/p21

    Plasmid

    #120232

    Purpose

    Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
    

    Use

    Synthetic Biology

    Mutation

    aac(3)IV (apramycin resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-I-SceI-Trex2

    Plasmid

    #44024

    Depositor

    Jeremy Stark

    Article

    Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

    Insert

    I-SceI fused to mTREX2
    

    Tags

    NLS-HA

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDNR-SpCas9-Gem

    Plasmid

    #80424

    Purpose

    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Gem
    

    Use

    CRISPR

    Tags

    Geminin

    Mutation

    codon optimized for human

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDNR-SpCas9-Cdt1

    Plasmid

    #80426

    Purpose

    Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Cdt1
    

    Use

    CRISPR

    Tags

    Cdt1

    Mutation

    codon optimized for human

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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