We narrowed to 79,188 results for: rest
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pBV-Luc
Plasmid#16539Purposeluciferase reporter plasmid with very low basal activityDepositorPublicationTypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pME-Cas9-T2A-GFP
Plasmid#63155PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.DepositorInsertCas9-T2A-GFP
UseCRISPR; Tol2 middle entry vector for gatewayAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cyto-roGFP
Plasmid#49435PurposeExpresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cellsDepositorInsertroGFP2
UseAdenoviralExpressionMammalianMutationC48S, Q80R, S147C, and Q204C in Clontech's G…PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSANG10-3F-BG4
Plasmid#55756PurposeExpresses BG4 scFv anti-G-quadruplex antibody in bacteriaDepositorInsertBG4
Tags6xHis, pelB leader, and tri-FLAGExpressionBacterialPromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pwtCas9-bacteria
Plasmid#44250PurposeaTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdownDepositorInsertwild-type Cas9
UseCRISPRExpressionBacterialPromoterpLtetO-1Available sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BFP-KDEL
Plasmid#49150Purposevisualization of the ERDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBiP ER signal sequence (HSPA5 Human)
TagsKDEL and TagBFPExpressionMammalianPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMIG-FLAG-MLL-AF9
Plasmid#71443PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFPDepositorAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-mGFP-Actin
Plasmid#21948DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbetaActin (ACTB Human)
TagsmEGFPExpressionMammalianAvailable sinceSept. 3, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEP4 E02S EM2K
Plasmid#20923Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseEpisomal/ebnaAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCALNL-GFP
Plasmid#13770PurposeCre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.DepositorInsertEGFP
UseCre/LoxExpressionMammalianPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCh-Rab7A
Plasmid#61804PurposeHuman Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab7A (RAB7A Human)
TagsmChExpressionMammalianMutationNonePromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Stat3-C Flag pRc/CMV
Plasmid#8722DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStat3-C (Stat3 Mouse)
TagsFlagExpressionMammalianMutationA662C and N664C. Residues changed to Cys renders…Available sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SIRT6 Flag
Plasmid#13817PurposeMammalian expression of human SIRT6 with flag tagDepositorPublicationAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ES-FUCCI
Plasmid#62451PurposeSMX_DEST vector with 3 cassettesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsminPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA
UseSynthetic BiologyAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-Ubiquitin-K11
Plasmid#22901DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationK11 only, all other lysines mutated to argininesAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ISceI-GR-RFP
Plasmid#17654DepositorTagsmRFPExpressionMammalianMutationC655G mutation in the glucocorticoid receptor (se…Available sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3XFlagNICD1
Plasmid#20183DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNotch 1 Intracellular Domain (Notch1 Mouse)
Tags3XFLAGExpressionMammalianMutationContains murine Notch 1 Intracellular Domain (Val…PromoterCMVAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS513 EF1alpha-cre
Plasmid#11918PurposeExpresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.DepositorPublicationAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG2LMKOSimO
Plasmid#20866DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange (Myc Mouse)
ExpressionMammalianAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by
