We narrowed to 19,002 results for: Tec

1. pCaRNA3

   Plasmid

   #86088

   Purpose

   Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   RNA3
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB411

   Plasmid

   #74805

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBiFC-bJunVN173

   Plasmid

   #22012

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   bJunVN173 (Jun Aequorea Victoria, Rat)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   c-Jun(257-338)-Venus(1-172)

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. TAL2289

   Plasmid

   #36724

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FANCG-TALEN_Right (Fancg Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pPAGFP-VSVG

   Plasmid

   #11915

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

   Insert

   VSVG
   

   Expression

   Mammalian

   Mutation

   (L64F/T65S/V163A/T203H). The PAGFP also contains …

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNS35 (multiAsCas12a piggyBac)

   Plasmid

   #217336

   Purpose

   piggyBac expression of multiAsCas12a

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pASKt15C+SrtA7

   Plasmid

   #64986

   Purpose

   Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Tags

   His6 tag and S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, P94R, E105K, E108A, D16…

   Promoter

   tetracycline promoter/operator

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX-evoCas9

   Plasmid

   #107550

   Purpose

   Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

   Depositor

   Anna Cereseto

   Article

   Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

   Insert

   Humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   M495V/Y515N/K526E/R661Q

   Promoter

   CBh

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGK111

   Plasmid

   #228126

   Purpose

   Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
   

   Expression

   Mammalian

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK7WGF2::hCas9

    Plasmid

    #46965

    Purpose

    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    hCas9
    

    Use

    CRISPR; Plant expression

    Tags

    eGFP

    Promoter

    35S

    Available Since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-Sp-gRNA-CCR5_DF_B584b_attB

    Plasmid

    #182148

    Purpose

    To insert attB attachment site at CCR5 in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    CCR5-B584b-attB pegRNA
    

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-Sp-gRNA-CCR5_DF_A509b_attB

    Plasmid

    #182147

    Purpose

    To insert attB attachment site at CCR5 in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    CCR5-A509b-attB pegRNA
    

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. yTREX

    Plasmid

    #177306

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

    Depositor

    Karl-Erich Jaeger

    Article

    Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Expression

    Bacterial

    Available Since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LbABE8e(TadA-8e V106W)

    Plasmid

    #138508

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-dLbCas12a
    

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWB511

    Plasmid

    #74853

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_123

    Plasmid

    #96919

    Purpose

    for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    p300

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

    Plasmid

    #169524

    Purpose

    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB417

    Plasmid

    #74811

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD/His-B-iRFP

    Plasmid

    #31855

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Insert

    iRFP
    

    Expression

    Bacterial

    Mutation

    S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…

    Promoter

    araBAD

    Available Since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJL-SaKKH-BE3

    Plasmid

    #85170

    Purpose

    Expresses SaKKH-BE3 in mammalian cells [Edit]

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    SaKKH-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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