We narrowed to 8,942 results for: itch
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Plasmid#53095PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.DepositorInsertLOV(wt)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in VenusAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mPA-GFP-PA-Rac1
Plasmid#22022PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6X His and mPA-GFPExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(fast)
Plasmid#107690PurposeN-terminal half of π-EB1 with fast LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
TagsA. sativa phototropin 1 LOV2 domain (I427V, H519L…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-BRD4ΔN-miRFP670-Cry2WT
Plasmid#122439PurposeExpresses disordered protein BRD4(462-1362) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertBRD4 (BRD4 Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationDeleted amino acids 1-461PromoterSFFVAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRY2-INPP5E
Plasmid#79562Purposeexpression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motifDepositorInsertINPP5E (INPP5E Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRY2-INPP5E(D556A)
Plasmid#79564Purposeexpression of CRY2PHR fused to catalytically dead INPP5EDepositorInsertINPP5E (INPP5E Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1-T17N
Plasmid#22017PurposeExpression of photoactivatable Rac1 (dominant-negative mutant)DepositorInsertPA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
Tags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutation T17N.PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
PEX-mCherry-CRY2
Plasmid#102250PurposeExpresses fusion of CRY2PHR with mCherry and PEX3 (1-42)DepositorTags6xHisExpressionMammalianPromoterCMVAvailable SinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
LIC1B_Caspase-LOVN7C-7 C450A
Plasmid#104628Purposebacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutationDepositorInsertCaspase-3 (CASP3 Human)
TagsHis and LOV2ExpressionBacterialMutationC450A (dark-locked and light-insensitive mutation)PromoterT7Available SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-hTRF2-tagRFP-T
Plasmid#103812PurposeBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M
Plasmid#22034DepositorInsertmVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))
UseRetroviralExpressionMammalianMutationThe LOV domain contains the mutation C450M.Rac1 s…Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID
Plasmid#122982PurposeAAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNTDepositorInsertmCh-IRES-BoNT/B(1-146)-iLID
UseAAVTagsmChExpressionMammalianMutationiLID contains long lived V416I mutationPromoterhuman synapsinAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A
Plasmid#22023DepositorInsertmVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
UseRetroviralExpressionMammalianMutationThe LOV domain contains the mutation C450A.Rac1 s…Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry GRK3 ct in pcDNA3.1
Plasmid#64213PurposemCherry fused between Cry2 and GRK3 ct to study cell migrationDepositorTagsmcherryExpressionMammalianPromoterCMVAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-FUSN-miRFP670-Cry2WT
Plasmid#122441PurposeExpresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertFUS (FUS Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationContains only amino acids 1-214PromoterSFFVAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mPA-GFP-PA-Rac1
Plasmid#22032DepositorInsertmPA-GFP-PA-Rac1 (RAC1 Human, Avena sativa (oat))
UseRetroviralExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
ECON
Plasmid#82553PurposeEpigenetic effector construct for targeted methylationDepositorTagsHALO and mCherryExpressionMammalianMutationN-terminal photolyase homology region domain of C…PromoterCMVAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hNCL
Plasmid#103805PurposeBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationhNCL: A395G (K132R), A421G (K141E), A424G (K142E)…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hNCL
Plasmid#103806PurposeBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationhNCL: A395G (K132R), A421G (K141E), A424G (K142E)…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi-dsm
Plasmid#89743PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11DepositorInsertNLS-OptoJNKi dark-state mutant
UseFluorescent proteinTagsmCherryExpressionMammalianMutationC450A mutation of AsLov2JalphaPromoterCMVAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only