We narrowed to 5,842 results for: pCas

1. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

   Plasmid

   #216733

   Purpose

   Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase and sgCTG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CAG

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGMC00027

   Plasmid

   #199279

   Purpose

   SpCas9 construct to knockout murine Cd274

   Depositor

   Raj Chari

   Article

   Ruf et al Cell. 2023 Aug 17;186(17):3686-3705.e32.

   Insert

   Cd274 guide RNA (Cd274 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCX-TRI-2A

   Plasmid

   #74674

   Purpose

   mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

   Depositor

   Roberto Giovannoni

   Article

   Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

   Inserts

   HO1 (HMOX1 Human)
   CD73 (NT5E Human)
   CD39 (ENTPD1 Human)
   

   Tags

   F2A

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. P3-dCas9-Tet1-GFP-Puro

   Plasmid

   #190728

   Purpose

   Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   3XFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   pCAG

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GCN5 core-dCas9-p300 core

   Plasmid

   #179553

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

   Plasmid

   #163643

   Purpose

   Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON17 hgRNA
   Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
   

   Use

   Lentiviral

   Tags

   Luc2-P2A-tdTomato-T2A

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   EFS and pU6

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GCN5 core-dCas9-CBP core

   Plasmid

   #179555

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. GCN5 FL-dCas9-CBP core

   Plasmid

   #179556

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GCN5 FL-dCas9-p300 core

   Plasmid

   #179554

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRISPRecise Kit

    Plasmid Kit

    #1000000235

    Purpose

    CRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.

    Depositor

    Ervin Welker

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

11. CRISPaint Gene Tagging Kit

    Plasmid Kit

    #1000000086

    Purpose

    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags

    Depositor

    Veit Hornung

    Available Since

    Sept. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

12. Diatom uLoop Assembly Kit

    Plasmid Kit

    #1000000238

    Purpose

    The collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.

    Depositor

    Christopher Dupont

    Available Since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

13. The Expanded CRISPEY Kit

    Plasmid Kit

    #1000000257

    Purpose

    Use the Expanded CRISPEY Kit for CRISPR-based gene editing of S. cerevisiae.

    Depositor

    Jeffrey Lewis

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

14. CyanoGate Kit

    Plasmid Kit

    #1000000146

    Purpose

    The CyanoGate kit contains 96 parts and acceptor vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.

    Depositor

    Alistair McCormick

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

15. Ubiquiton Yeast Kit

    Plasmid Kit

    #1000000243

    Purpose

    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.

    Depositor

    Helle Ulrich

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

16. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit

    #1000000236

    Purpose

    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.

    Depositor

    Daniel Voytas

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

17. Cultivarium POSSUM Toolkit

    Plasmid Kit

    #1000000234

    Purpose

    The POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.

    Depositor

    Nili Ostrov

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

18. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit

    #1000000030

    Purpose

    Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.

    Depositor

    Takashi Yamamoto

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

19. Rinehart & Söll Phosphoprotein Synthesis Kit

    Plasmid Kit

    #1000000020

    Purpose

    Allows Sep incorporation (directed by UAG) into a protein by an E. coli strain that harbors a Sep-accepting tRNA (tRNASep), its cognate Sep–tRNA synthetase (SepRS), and an engineered EF-Tu (EF-Sep).

    Depositor

    Jesse Rinehart, Dieter Söll

    Available Since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Yeast Secrete and Detect

    Plasmid Kit

    #1000000166

    Purpose

    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.

    Depositor

    Sylvestre Marillonnet

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only