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pNDGG057

Plasmid

#231327

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RSF1010-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pNDGG005, pOGG012, and pOGG014, NmR/KmR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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3xmScarletI_UtrCH

Plasmid

#112962

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

UtrCH

Tags

3x mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-TMEM30A-V5

Plasmid

#225510

Purpose

TMEM30A expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM30A (TMEM30A Human)

Tags

V5

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-C1-P4Mx2

Plasmid

#202681

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PAmCherry1: L. pneumophila SidM(546-647):SidM(546-647) (sidM Legionella pneumophila)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRTH

Plasmid

#136925

Purpose

Expresses Cas9-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Zeb2-3'UTR

Plasmid

#61771

Purpose

Luciferase reporter containing the 3'UTR of mouse Zeb2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zeb2 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
EB3-3xmNeonGreen-N1

Plasmid

#129605

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-SIV-MAC251-vpx

Plasmid

#115842

Purpose

Encodes codon optimized FLAG tagged SIVMAC251-Vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251-Vpx

Tags

3x-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PX552-mScn2a-3xV5-EF1-smFP-flag

Plasmid

#182561

Purpose

For mouse Nav1.2 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn2a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNTI661 pRPR1(TetO)-sgRNA

Plasmid

#139475

Purpose

Vector for tet-regulated sgRNA expression in yeast

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Insert

single guide RNA scaffold

Use

CRISPR

Expression

Yeast

Promoter

pRPR1(TetO)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag WDR5

Plasmid

#15552

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Wdr5 (Wdr5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

Plasmid

#55222

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pP35S (GB0030)

Plasmid

#68163

Purpose

Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV 35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRCH

Plasmid

#136926

Purpose

Expresses Cas9-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Sa-SauriCas9

Plasmid

#135967

Purpose

Expresses Sa-SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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TRAM1-EGFP

Plasmid

#210365

Purpose

Expresses TRAM1 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TRAM1 (TRAM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-C1-P4C

Plasmid

#202680

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PAmCherry1: L. pneumophila SidC (608-773) (sidC Legionella pneumophila)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-VPS15-TSF

Plasmid

#203563

Purpose

Mammalian expression of VPS15 with TwinStrep-Flag tag

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

VPS15 (PIK3R4 Human)

Tags

TwinStrep Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA6

Plasmid

#19486

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 6 (HSPA6 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato

Plasmid

#125573

Purpose

Cre-dependent expression of tdTomato in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

tdTomato

Use

Cre/Lox

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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