Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 10,128 results for: Met

Clear keywords
Showing: 5961 - 5980 of 10128 results

  3. pQCXIB Flag MFF S155A

    Plasmid
    #74385
    Purpose
    Retroviral expression vector containing Flag tagged S155A MFF isoform 5 mutant
    Depositor
    Reuben Shaw
    Publication
    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d
    Insert
    Flag-MFF (MFF Human)
    Use
    Retroviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Ser 155 Ala (numbering based on MFF isoform 1)
    Promoter
    CMV
    Available since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. pQCXIB Flag MFF S172A

    Plasmid
    #74386
    Purpose
    Retroviral expression vector containing Flag tagged S172A MFF isoform 5 mutant
    Depositor
    Reuben Shaw
    Publication
    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d
    Insert
    Flag-MFF (MFF Human)
    Use
    Retroviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Ser 172 Ala (numbering based on MFF isoform 1)
    Promoter
    CMV
    Available since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. mutpRL-TK 3xUTR

    Plasmid
    #40758
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    3 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pJRoC30_Th3.10

    Plasmid
    #188067
    Purpose
    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)
    Depositor
    Sarel Fleishman
    Publication
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    20 PROSS + 3 FuncLib mutations, described in publ…
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188067-plasmid-map-sequence-id-370135

    Enlarge

  9. pJRoC30_Th3.1

    Plasmid
    #188065
    Purpose
    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)
    Depositor
    Sarel Fleishman
    Publication
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    20 PROSS + 4 FuncLib mutations, described in publ…
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188065-plasmid-map-sequence-id-370134

    Enlarge

  10. pJRoC30_Th3.7

    Plasmid
    #188066
    Purpose
    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.7)
    Depositor
    Sarel Fleishman
    Publication
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    20 PROSS + 3 FuncLib mutations, described in publ…
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188066-plasmid-map-sequence-id-370143

    Enlarge

  11. pFLAG-CMV2-MLL4fusion

    Plasmid
    #255997
    Purpose
    This plasmid expresses a mini-version of human MLL4 (aka KMT2D), which is composed of a tandem PHD (PHD4-6;1358–1572aa) and a SET (catalytic domain)-containing C-terminal region (4507–5537aa).
    Depositor
    Min Gyu Lee
    Publication
    Dhar et al Genes Dev. 2012 Dec 15;26(24):2749-62. d
    Insert
    MLL4 fusion (KMT2D Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    MLL4fusion with PHD(4-6) 1358–1572 and SET (catal…
    Promoter
    CMV
    Available since
    June 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    255997-plasmid-map-sequence-id-513255

    Enlarge

  16. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

    Plasmid
    #174633
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Publication
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
    Tags
    mCherry-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174633-plasmid-map-sequence-id-346989

    Enlarge

  17. Epac-S-H150

    Plasmid
    #170345
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170345-plasmid-map-sequence-id-344646

    Enlarge

  18. Epac-S-H94

    Plasmid
    #170341
    Purpose
    GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170341-plasmid-map-sequence-id-335921

    Enlarge

  19. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid
    #129053
    Purpose
    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129053-plasmid-map-sequence-id-256238

    Enlarge

  20. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129036
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA8 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129036-plasmid-map-sequence-id-256165

    Enlarge

Showing: 5961 - 5980 of 10128 results