We narrowed to 14,600 results for: Ott

1. pOTTC414 - pAAV EF1a hPREP

   Plasmid

   #59967

   Purpose

   An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOTTC841 - pAAV minP secNLuc

   Plasmid

   #82498

   Purpose

   Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

   Depositor

   Brandon Harvey

   Article

   Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

   Insert

   secNLuc
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   c-Fos minimal promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOTTC592 - pCMV Mito-iRFP

   Plasmid

   #59135

   Purpose

   A plasmid that express mitochondrial-targeted iRFP under the CMV promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Mitochondria-localized Infrared Fluorescent Protein
   

   Use

   Tags

   Mitochondria-targeting sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOTTC591 - pCMV Mem-iRFP

   Plasmid

   #59134

   Purpose

   A plasmid that express membrane-targeted iRFP under the CMV promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Membrane-localized Infrared Fluorescent Protein
   

   Use

   Tags

   Palmitylation signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOttc1495 - pAAV CaMKII SERCaMP_ASARTDL

   Plasmid

   #192602

   Purpose

   An AAV packaging vector that expresses SERCaMP under control of the CaMKII promoter.

   Depositor

   Brandon Harvey

   Insert

   SERCaMP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII

   Available since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLKO5-SgHottip-GFP-CRISPRa

   Plasmid

   #134990

   Purpose

   dCas9-mediated activation of HOTTIP in mammalian cells

   Depositor

   Suming Huang

   Article

   Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

   Insert

   HOTTIP (HOTTIP Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19-P70a-Top-T~Bottom-T

   Plasmid

   #87315

   Purpose

   Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system (promoter control)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOttc556 - pAAV EF1a iCre

   Plasmid

   #210416

   Purpose

   AAV viral vector packaging plasmid that expresses "improved Cre recombinase"

   Depositor

   Brandon Harvey, Christopher Richie

   Insert

   iCre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD

   Plasmid

   #201818

   Purpose

   A plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoter

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   V5-ER-LBD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO5-SgHottip-GFP-CRISPRi

    Plasmid

    #134989

    Purpose

    dCas9-mediated inactivation of HOTTIP in mammalian cells

    Depositor

    Suming Huang

    Article

    Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

    Insert

    HOTTIP (HOTTIP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

    Plasmid

    #113163

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

    Plasmid

    #47904

    Purpose

    An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    eNpHR3.0
    

    Use

    AAV

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)

    Plasmid

    #125146

    Purpose

    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

    Depositor

    Christopher Richie

    Insert

    HA-tagged hM4D(Gi) (CHRM4 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SYN1

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOTTC374 - pAAV EF1a DIO iRFP

    Plasmid

    #47626

    Purpose

    An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Infrared fluorescent protein
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOTTC385 - pAAV CMV-IE IRES EGFP

    Plasmid

    #102936

    Purpose

    An AAV vector that expresses IRES EGFP under the CMV-IE promoter

    Depositor

    Brandon Harvey

    Article

    Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid

    #113161

    Purpose

    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat Rosa26
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOTTC589 - pAAV c-fos Nuc-eYFP

    Plasmid

    #59133

    Purpose

    An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Nuclear-localized Enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    mFos

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOTTC337 - pAAV EF1a DIO mCherry

    Plasmid

    #47636

    Purpose

    An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOTTC1761 - pAAV SYN1 DIO HA-hM3D(Gq)

    Plasmid

    #125147

    Purpose

    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM3D(Gq)) from a synapsin promoter

    Depositor

    Christopher Richie

    Insert

    HA-tagged hM3D(Gq) (CHRM3 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SYN1

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

    Plasmid

    #112719

    Purpose

    An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Mecp2

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOTTC588 - pAAV c-fos Nuc-iRFP

    Plasmid

    #59132

    Purpose

    An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Nuclear-localized Infrared Fluorescent Protein
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    mFos

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pOTTC446 - pAAV EF1a hPREP(S554A)

    Plasmid

    #59968

    Purpose

    An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    Prolyl oligopeptidase (PREP Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S554A

    Promoter

    EF1a

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOTTC1553 - pAAV EF1a EGFP-KASH

    Plasmid

    #131682

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Insert

    EGFP-KASH
    

    Use

    AAV

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

    Plasmid

    #75295

    Purpose

    An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.

    Depositor

    Brandon Harvey

    Insert

    eGFP-2A-mKv1.2 (Kcna2 Synthetic, Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human SYN1

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Anti-Ankyrin-G (blotting) [N106/20R]

    Plasmid

    #177495

    Purpose

    Mammalian Expression Plasmid of anti-Ankyrin-G (blotting) (Human). Derived from hybridoma N106/20.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Ankyrin-G (blotting) (Homo sapiens) recombinant mouse monoclonal antibody (ANK3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTre-Tight-luciferase-Hottip (522)

    Plasmid

    #65490

    Purpose

    positive control for lncRNA expression assay

    Depositor

    Roderic Guigo, Rory Johnson

    Insert

    Hottip (HOTTIP Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC19-P70a-Top-T~P70a-Bottom-T

    Plasmid

    #87314

    Purpose

    Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOTTC1617 pAAV SYN1 Nuc-EYFP

    Plasmid

    #135567

    Purpose

    An AAV vector expressing a neuronally expressed nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    empty
    Nuc-EYFP
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN1

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOttc1494 - pAAV CaMKII SERCaMP-No Tag

    Plasmid

    #192601

    Purpose

    An AAV packaging vector that expresses SERCaMP under control of the CaMKII promoter.

    Depositor

    Brandon Harvey

    Insert

    SERCaMP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVH229-Tier1-OTtgO2-PCMVmin2-SEAP

    Plasmid

    #169565

    Purpose

    Tier-1 vector encoding PTtgO2-driven SEAP expression (OTtgO2-PCMVmin-2-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    phloretin-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TtgO2-PCMVmin

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

    Plasmid

    #113159

    Purpose

    An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP

    Plasmid

    #47631

    Purpose

    An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    eNpHR3.0
    

    Use

    AAV and Cre/Lox

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

    Plasmid

    #112683

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-iCre and Nuclear localization signal

    Expression

    Mutation

    Promoter

    CMV-IE

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

    Plasmid

    #75082

    Purpose

    An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Nuc-eYFP
    

    Use

    AAV and Cre/Lox

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

    Plasmid

    #102935

    Purpose

    An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

    Depositor

    Brandon Harvey

    Article

    Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

    Insert

    NK1R (Tacr1 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

    Plasmid

    #112684

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-Flpo and Nuclear localization signal

    Expression

    Mutation

    Promoter

    CMV-IE

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

    Plasmid

    #47905

    Purpose

    An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pOTTC710 - pAAV EF1a DIO Mem-AcGFP

    Plasmid

    #75081

    Purpose

    An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Mem-AcGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitylation site

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63886

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human SYN1

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

    Plasmid

    #113162

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

    Plasmid

    #113156

    Purpose

    An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat Rosa26
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63884

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

    Plasmid

    #113155

    Purpose

    An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

    Plasmid

    #113157

    Purpose

    An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat MANF
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pOttc1472 - pAAV CaMKII KDELR1-Myc-DDK

    Plasmid

    #192599

    Purpose

    An AAV packaging vector that expresses KDELR1 under control of the CaMKII promoter.

    Depositor

    Brandon Harvey

    Insert

    KDELR1 (KDELR1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP

    Plasmid

    #47631

    Purpose

    An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    eNpHR3.0
    

    Use

    AAV and Cre/Lox

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

    Plasmid

    #112683

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-iCre and Nuclear localization signal

    Expression

    Mutation

    Promoter

    CMV-IE

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

    Plasmid

    #75082

    Purpose

    An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Nuc-eYFP
    

    Use

    AAV and Cre/Lox

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pOttc438 - pAAV EF1a CRE ERT2 bGHpA

    Plasmid

    #210415

    Purpose

    AAV viral vector packaging plasmid that expresses tamoxifen-regulated Cre recombinase

    Depositor

    Brandon Harvey, Christopher Richie

    Insert

    Cre-ERT2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

    Plasmid

    #102935

    Purpose

    An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

    Depositor

    Brandon Harvey

    Article

    Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

    Insert

    NK1R (Tacr1 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge