We narrowed to 26,060 results for: Spr;

1. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

   Plasmid

   #41863

   Purpose

   This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

   Plasmid

   #62577

   Purpose

   Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

   Plasmid

   #62580

   Purpose

   Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Mutation

   miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

   Plasmid

   #62579

   Purpose

   Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

   Plasmid

   #62581

   Purpose

   Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Mutation

   miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Jun lab tunable CRISPRi strain

   Bacterial Strain

   #86400

   Purpose

   Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

   Depositor

   Suckjoon Jun

   Article

   Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

   Bacterial Resistance

   Spectinomycin and hygromycin

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

7. SPR

   Plasmid

   #39161

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. lentiCRISPRv2FE-ABE8e-SpRY

   Plasmid

   #213008

   Purpose

   A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAEL010313 SPR

   Plasmid

   #115385

   Purpose

   Expresses Ae. aegypti receptor in mammalian cells

   Depositor

   Leslie Vosshall

   Article

   Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

   Insert

   G-protein coupled receptor daf-37
   

   Expression

   Mammalian

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR v2

    Plasmid

    #52961

    Purpose

    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPRv2GFP

    Plasmid

    #82416

    Purpose

    3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

    Depositor

    David Feldser

    Article

    Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS (P2A)

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPR

    Plasmid

    #42875

    Purpose

    A crRNA expression plasmid for targeting a specific sequence.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR-BsaI
    

    Use

    CRISPR; E.coli

    Expression

    Bacterial

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENN236_CRISPRcharm_Kv2

    Plasmid

    #220839

    Purpose

    CRISPRcharm Kv2 expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm Kv2
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACRISPR

    Plasmid

    #113348

    Purpose

    A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. OpenCRISPR-1

    Plasmid

    #221565

    Purpose

    Express OpenCRISPR-1 nuclease in human cells using a CMV promoter

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. p101_CRISPRai_PiggyBac

    Plasmid

    #213777

    Purpose

    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    

    Use

    CRISPR

    Promoter

    EF1a (EF-1-alpha intron a) and Tet_On_TRE

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-PE SpRY_ATH51

    Plasmid

    #235998

    Purpose

    Expresses SpRY_ATH51 prime editor and pegRNA in mammalian cells

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    SpRY_ATH51_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    bpSV40 NLS

    Expression

    Mammalian

    Mutation

    ectopic insertion of 7 amino acids of PID turn-he…

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENN226_CRISPRcharm

    Plasmid

    #220834

    Purpose

    CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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