Addgene

We narrowed to 17,038 results for: URE

Showing: 41 - 60 of 17038 results

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Anti-CASPR/Neurexin IV [K65A/30R]

Plasmid

#219448

Purpose

Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat) IgG2a R-mAb. Derived from hybridoma K65A/30.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cntnap1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-CASPR/Neurexin IV [K65A/43R]

Plasmid

#219449

Purpose

Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat) IgG2a R-mAb. Derived from hybridoma K65A/43.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cntnap1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-CASPR/Neurexin IV [K65/35R-1]

Plasmid

#206683

Purpose

Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant Mouse monoclonal antibody (Cntnap1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-CASPR/Neurexin IV [K65/35R-2b]

Plasmid

#199403

Purpose

Mammalian expression plasmid of Anti-CASPR/Neurexin IV [K65/35R-2b] (Rat). Derived from hybridoma K65/35.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-CASPR/Neurexin IV (Rat) recombinant mouse monoclonal antibody (Cntnap1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-Unstructured-EIF3B-465-552

Plasmid

#136029

Purpose

EIF3B (465-552) 3'UTR with the Artificial Unstructured 3'UTR fused upstream inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-465-552-Unstructured

Plasmid

#136028

Purpose

EIF3B (465-552) 3'UTR with the Artificial Unstructured 3'UTR fused downstream inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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LOC5574680 Atrial natriuretic peptide receptor

Plasmid

#115374

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5574680 Atrial natriuretic peptide receptor (LOC5574680 Aedes aegypti)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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RalA (G23V)(mature peptide)-pcw107-V5

Plasmid

#64643

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

G23V

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
RalA (G23V)(mature peptide)-pcw107

Plasmid

#64642

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

Expression

Mutation

G23V

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

Plasmid

#138418

Purpose

CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Insert

dCas9

Use

Lentiviral

Tags

BioTAP-tag

Expression

Mutation

Promoter

EF1a

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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His-TEV-Aurein1.2_(+36GFP)-LPETG-His

Plasmid

#71755

Purpose

His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2_(pos36_GFP)-LPETG-His

Use

Tags

6XHis, Sortase (LPETG), and TEV Protease

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

Plasmid

#123614

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A)-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1

Plasmid

#138419

Purpose

CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Insert

dCas9

Use

Lentiviral

Tags

BioTAP-tag

Expression

Mutation

Promoter

EF1a

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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mature ISG15 C-term. Domain (79-165, W123A)

Plasmid

#110765

Purpose

Bacterial expression of mutant proISG15 C-term. domain

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

proISG15 C-term. domain (79-165, W123A) (ISG15 Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

W123A

Promoter

T7

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

Plasmid

#123615

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A/K34A)-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

R33A/K34A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Neurexin-1-Beta [N170A/1-rat IgG2a] - Chimeric

Plasmid

#227063

Purpose

Mammalian expression plasmid of anti-Neurexin-1-Beta (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma N170A/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Neurexin-1-Beta (Homo sapiens) recombinant mouse monoclonal antibody. (NRXN1 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-CASPR/Neurexin IV [K65/35R-rat IgG2a]

Plasmid

#222178

Purpose

Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cntnap1 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

Plasmid

#138417

Purpose

CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Inserts

BirA
dCas9

Use

Lentiviral

Tags

FLAG, Avi-tag and V5, His

Expression

Mutation

Promoter

EF1a

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold

Plasmid

#186981

Purpose

mammalian expression of Cas7-11 crRNA guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

mature DR guide

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#213480

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA-directcapture

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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