Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 3,499 results for: multiplex

Clear keywords
Showing: 41 - 60 of 3499 results

  5. pRGEB32

    Plasmid
    #63142
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
    Depositor
    Yinong Yang
    Publication
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63142-plasmid-map-sequence-id-339931

    Enlarge

  10. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Publication
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60847-plasmid-map-sequence-id-197511

    Enlarge

  11. Puro-Cas9 donor

    Plasmid
    #58409
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus
    Depositor
    Danwei Huangfu
    Publication
    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58409-plasmid-map-sequence-id-392399

    Enlarge

  13. pAC93-pmax-dCas9VP160

    Plasmid
    #48225
    Purpose
    dCas9VP160 on pmax expression vector
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain
    Use
    CRISPR
    Tags
    HA Tag and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48225-plasmid-map-sequence-id-201375

    Enlarge

  14. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid
    #48226
    Purpose
    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    Use
    CRISPR
    Tags
    2A-puro, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48226-plasmid-map-sequence-id-69514

    Enlarge

  15. pHSE401

    Plasmid
    #62201
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62201-plasmid-map-sequence-id-228092

    Enlarge

  16. pYPQ152

    Plasmid
    #69303
    Purpose
    Gateway entry vector with pco-dCas9-VP64
    Depositor
    Yiping Qi
    Publication
    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pco-dCas9-VP64 (Plant codon-optimized)
    Use
    CRISPR
    Mutation
    VP64 activator fusion; NLS fusion
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69303-plasmid-map-sequence-id-247582

    Enlarge

  19. pKSE401

    Plasmid
    #62202
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62202-plasmid-map-sequence-id-288285

    Enlarge

Showing: 41 - 60 of 3499 results