We narrowed to 5,882 results for: pCas

1. pCas-CmR(+)

   Plasmid

   #113633

   Purpose

   Chloramphenicol resistant derivative of pCas plasmid

   Depositor

   Olivier Lichtarge

   Article

   Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

   Insert

   Chloramphenicol resistance gene
   

   Use

   CRISPR

   Promoter

   Pcat

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas-dCas12m

   Plasmid

   #192275

   Purpose

   Encodes MmdCas12m (D485A) under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D485A

   Promoter

   PJ23108

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA-FLEX

   Plasmid

   #38041

   Depositor

   Naoshige Uchida

   Article

   Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CA

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA-ZtTA

   Plasmid

   #26083

   Depositor

   Liqun Luo

   Article

   Li et al PLoS One. 2010 . 5(7):e11503.

   Insert

   a loxP-flanked β-geo preceding the tetracycline transactivator tTA
   

   Expression

   Mammalian

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas-mut4_1_2_VA

   Plasmid

   #186441

   Purpose

   Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4, Cas1 and Cas2
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Plasmid

   #101217

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

   Promoter

   T7

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRepCap6

   Plasmid

   #110770

   Purpose

   pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.

   Depositor

   David Russell

   Article

   Rutledge et al J Virol. 1998 Jan;72(1):309-19.

   Insert

   AAV6 cap, AAV6 rep genes
   

   Use

   AAV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas3cRh

   Plasmid

   #133773

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Joseph Bondy-Denomy

   Article

   Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   Cas7
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCasSA

   Plasmid

   #98211

   Purpose

   CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

   Depositor

   Quanjiang Ji

   Article

   Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9

    Plasmid

    #42876

    Purpose

    Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    tracr/Cas9
    

    Use

    CRISPR; E.coli

    Expression

    Bacterial

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas12f1

    Plasmid

    #171182

    Purpose

    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    Cas12f1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    cas9 promoter from Streptococcus pyogenes

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGIG

    Plasmid

    #11159

    Purpose

    Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    IRES-EGFP
    

    Expression

    Mammalian

    Available Since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG_eGCaMP(2+)

    Plasmid

    #201148

    Purpose

    expresses eGCaMP2+ in mammalian cells

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP2+
    

    Expression

    Mammalian

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCasTet-λ

    Plasmid

    #128318

    Purpose

    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

    Depositor

    Christopher Johnston

    Article

    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

    Insert

    TetR and pTetR/TetO
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    Constitutive expression of cas9 and anhydro-tetra…

    Promoter

    tet promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9_GFP

    Plasmid

    #44719

    Purpose

    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAH01

    Plasmid

    #128162

    Purpose

    IncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteria

    Depositor

    Michael Guarnieri, Calvin Henard

    Article

    Henard et al Sci Rep. 2016 Feb 23;6:21585. doi: 10.10

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Expression

    Bacterial

    Promoter

    prab17

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS_cAMPFIRE-L

    Plasmid

    #182283

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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