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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,131 results for: HL

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Showing: 581 - 600 of 1131 results

  6. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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  9. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Article
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available Since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119816-plasmid-map-sequence-id-372950

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  11. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available Since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

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  13. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

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Showing: 581 - 600 of 1131 results