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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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amilCP chromoprotein

Plasmid

#117847

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180267

Purpose

Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

EGFR sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo

Plasmid

#25735

Purpose

3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pVSVΔG-eGFP-linker

Plasmid

#231707

Purpose

Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

Depositor

Ron Geller

Article

Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

Insert

VSV antigenome

Use

Vsv expression

Expression

Mammalian

Mutation

ΔG (VSV glycoprotein removed)

Promoter

T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5R 15xGly

Plasmid

#211355

Purpose

Transient expression of 15xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-EGFP-3xHA

Tags

EGFP-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4

Plasmid

#29527

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

Wild Type

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pUB-GFP

Plasmid

#11155

Purpose

Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

Ubiquitin C promoter (UBC Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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eGFP-MYO5B

Plasmid

#239265

Purpose

Expression of N terminal GFP tagged Myo5B

Depositor

David Pellman

Article

Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

Insert

MYO5B (MYO5B Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180268

Purpose

Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

GARP sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Split random

Plasmid

#200646

Purpose

Expressed a eGFP sequence where a chimeric intron with a random sequence was inserted

Depositor

Claire Francastel

Article

Bogard et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

Split GFP with chimeric intron containing random sequence

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-BFP

Plasmid

#78545

Purpose

Fusion protein Cas9+BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

BFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-V106W

Plasmid

#215825

Purpose

Express CBE6b V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCAX Itgb1-FLAG

Plasmid

#30153

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

integrin beta 1 (Itgb1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pCAGGS DRR mCherry Donor EF1a BFP

Plasmid

#98896

Purpose

Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-mCherry-GFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-BFP

Plasmid

#78547

Purpose

Cas9 protein with BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-6

Plasmid

#163132

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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EED-KO-gRNA-1

Plasmid

#131321

Purpose

gRNA to knockout EED. Use with EED-KO-gRNA-2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-1

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
TRE-KRAB-dCas9-IRES-GFP

Plasmid

#85556

Purpose

2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-GFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TYMS

Plasmid

#100545

Purpose

mammalian expression of thymidylate synthetase

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidylate synthetase (TYMS Human)

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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