We narrowed to 11,576 results for: Vars;

1. pDONR223 SARS-CoV2 TEV-N

   Plasmid

   #154424

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   N (N SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available Since

   Sept. 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pPGK-T7/2-CD44v3 (-cyt)

   Plasmid

   #137813

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v3 (-cyt) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region, N280D in the V3 region

   Promoter

   PGK

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBT-RP8.1

   Plasmid

   #189846

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   mRFP and tagBFP

   Expression

   Mutation

   Promoter

   beta Actin

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139095

   Purpose

   Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dSpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMS1626

   Plasmid

   #29281

   Purpose

   The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple155-Intron-LacZ-NLS
   

   Use

   High copy number homologous recombination plasmid…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MiniPromoter Ple155 from the human PCP2 gene

   Available Since

   June 10, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. ACTB-(A)3x

   Plasmid

   #112057

   Purpose

   ACTB mRNA tagged with 3 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   3 copies of Riboglow tag (variant A) after stop c…

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ACTB-(A)2x

   Plasmid

   #112056

   Purpose

   ACTB mRNA tagged with 2 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   2 copies of Riboglow tag (variant A) after stop c…

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223 SARS-CoV2 TEV-ORF3B

   Plasmid

   #154417

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF3B
   

   Use

   Gateway-compatible entry vector, with insert of o…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available Since

   July 7, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. AT1G66830 X005_pECIA14

   Plasmid

   #114810

   Purpose

   Prey vector AT1G66830 X005_pECIA14 should be used with bait vector AT1G66830 X005_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G66830 (AT1G66830 Mustard Weed)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106179

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    hSynapsin

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

    Plasmid

    #114088

    Purpose

    U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for MbCas12a crRNAs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIE-C-hα2M-H6

    Plasmid

    #128494

    Purpose

    Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (R787-N1473) (A2M Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    contains R787-N1473

    Promoter

    IE1

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Flag-RNF168 delta MIU2

    Plasmid

    #133979

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deleted

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of MIU2

    Promoter

    CMV

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDONR221 SARS-CoV-2 S-frag3_nostop

    Plasmid

    #153175

    Purpose

    Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth, Mikko Taipale

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S-frag3
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available Since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pDONR221 SARS-CoV-2 S-frag4_nostop

    Plasmid

    #153176

    Purpose

    Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth, Mikko Taipale

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S-frag4
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available Since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pDONR221 SARS-CoV-2 S-frag1_nostop

    Plasmid

    #153173

    Purpose

    Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth, Mikko Taipale

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S-frag1
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available Since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

    Plasmid

    #169467

    Purpose

    Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP N1 mitoAURKA

    Plasmid

    #157754

    Purpose

    Expression of AURKA:GFP targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    AURKA without the first 30 amino acids replaced b…

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His6-SMT3-SopB(C460S)

    Plasmid

    #183677

    Purpose

    Recombinant Salmonella Typhimurium SopB (catalytically inactive)

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
    

    Use

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Mutation

    C460S

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_TPTE_WT

    Plasmid

    #81867

    Purpose

    Gateway Donor vector containing TPTE , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TPTE (TPTE Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    L452P

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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