We narrowed to 34,882 results for: cas

1. pMT493

   Plasmid

   #139398

   Purpose

   Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Bile Acid inducible Promoter

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT494

   Plasmid

   #139399

   Purpose

   Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Bile Acid inducible Promoter

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT449

   Plasmid

   #139389

   Purpose

   Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Bile Acid inducible Promoter

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMT451

   Plasmid

   #139391

   Purpose

   Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BA inducible promoters

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT455

   Plasmid

   #139392

   Purpose

   aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   aTC and BA inducible promoters

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. SQT1665

   Plasmid

   #78744

   Purpose

   Human expression plasmid for LbCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. SQT1659

   Plasmid

   #78743

   Purpose

   Human expression plasmid for AsCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRH623

   Plasmid

   #171633

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-non-targeting
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAT007

   Plasmid

   #171634

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TetO-FUW-VdC9BV

    Plasmid

    #62195

    Purpose

    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

    Depositor

    Kam Leong

    Article

    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

    Insert

    VP64dCas9BFPVP64
    

    Use

    Lentiviral

    Tags

    Two VP64s tagged to dCas9 fused to BFP

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive) Cas9 (dCas9)

    Promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTU422

    Plasmid

    #178982

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Inserts

    gRAMP
    CRISPR array
    

    Use

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT44c

    Plasmid

    #138511

    Purpose

    P2

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    inteinC-dSpCas9-UGI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJRH056

    Plasmid

    #171637

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-tdTomato
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2T-CAG-MCS-P2A-GFP-PuroR

    Plasmid

    #107186

    Purpose

    Base plasmid for cloning gene duplication alleles with eGFP reporter.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2T-CMV-eA3Amax-T31A-NG-BlastR

    Plasmid

    #153000

    Purpose

    C-to-T/G/A base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    eA3A-BE4 T31A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAT024

    Plasmid

    #171636

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BFP
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTU387

    Plasmid

    #178981

    Purpose

    pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    CRISPR array from Candidatus Scalindua brodae
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCT

    Plasmid

    #60620

    Purpose

    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL0373 (His-MBP-TniQ)

    Plasmid

    #130638

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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