176,133 results

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PB-tetO-hNIL

Plasmid

#197089

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHTNW

Plasmid

#136403

Purpose

Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPIDCB2

Plasmid

#169900

Purpose

Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Expression

Mammalian

Promoter

Dox inducible

Available Since

Dec. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUHD 10.3-human MIZ1 delta POZ

Plasmid

#74160

Purpose

Tet inducible expression of MIZ1 delta POZ

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 delta POZ (ZBTB17 Human)

Expression

Mammalian

Mutation

delta POZ domain (first 109 aa)

Promoter

CMV2

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only
TIRAP-GFP

Plasmid

#52739

Purpose

Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker

Depositor

Jonathan Kagan

Article

Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

Insert

Toll/IL-1 Receptor adaptor protein (TIRAP Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pDisplay-GRAB_g5-HT3.0

Plasmid

#208711

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in mammalian cells

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G466V

Plasmid

#131722

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G466V

Promoter

CMV

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEIGW roGFP2-Orp1

Plasmid

#64993

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR223-BALF4

Plasmid

#195835

Purpose

Gateway Entry Clone of Epstein-Barr virus BALF4

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF4 (BALF4 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-FOXO3a WT

Plasmid

#8360

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

FOXO3a (FOXO3 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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UMI-sgRNA-CH

Pooled Library

#234820

Purpose

Unique molecular identifiers-linked sgRNA library targeting chromatin factors (CH) in mice.

Depositor

Benjamin Ebert

Article

Loke et al Blood. 2025 Jun 10:blood.2024028033. doi

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
pJB127

Plasmid

#248630

Purpose

Theophylline-based control of repA on a C. difficile plasmid for use in allelic exchange and codA added to help cure strains of the plasmid.

Depositor

Joseph Sorg

Article

Brehm et al Anaerobe. 2024 Apr 29;88:102858. doi: 10

Inserts

sacB (sacB )
codA

Use

Other

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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MTS-EGFP-FLAG-APEX2

Plasmid

#251713

Purpose

Expresses mitochondrial EGFP-FLAG-APEX2 in mammalian cells

Depositor

Svetlana Konovalova

Insert

Mitochondrial targeting sequence (MTS) (APEX2 Human)

Tags

APEX2, EGFP, and FLAG

Expression

Mammalian

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pHAGE-KNL1-s24a_s60a-dCas9

Plasmid

#248568

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-s24a_s60a-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 S24A, S60A; Cas9 D10A, H840A

Promoter

CMV, TetO2

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCas9-EGFP_SLEEK

Plasmid

#249545

Purpose

Insert the Cas9-EGFP into exon 9 of GAPDH for stable expression.

Depositor

Joe Zhang

Article

Zhang et al STAR Protoc. 2025 Jul 4;6(3):103928. doi

Insert

5'arm-Exon9-Cas9-EGFP-3'arm

Use

CRISPR

Tags

EGFP

Promoter

U6 promoter

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a myc tagged mbIL15 IRES miRFP720-P2A-Blast

Plasmid

#248032

Purpose

Constitutively expresses myc tagged membrane bound IL15, miRFP720 and a blasticidin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

myc-tagged membrane bound IL15 (IL15 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC2

Plasmid

#183334

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC2

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA7-1

Plasmid

#248530

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA7-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA7-3

Plasmid

#248531

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA7-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA10-3

Plasmid

#248536

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA10-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-PA-GFP-H4-FRT

Plasmid

#247333

Purpose

Expresses histone H4 fused with photoactivatable-GFP

Depositor

Kazuhiro Maeshima

Article

Hihara et al Cell Rep. 2012 Dec 27;2(6):1645-56. doi:

Insert

H4 (H4C3) (H4C3 Human)

Tags

PA-GFP

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TTR A25T

Plasmid

#238114

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

A25T

Promoter

T7

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-eGFP-2A-mCherry_D4H

Plasmid

#194878

Purpose

Ratiometric D4H sensor for in vivo astrocyte-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_131

Plasmid

#225824

Purpose

Tn libraries Tn5 variant TET backbone

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Type

Empty backbone

Use

Synthetic Biology

Mutation

N/A

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro 3XFLAG-hATXN7L3

Plasmid

#225725

Purpose

Transfection of ATXN7L3 with 3XFLAG tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

ATXN7L3 (ATXN7L3 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF1647

Plasmid

#143303

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF358 (ZNF358 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pILGFP5EG3

Plasmid

#185878

Purpose

A plasmid for in vivo gene amplification (HapAmp) at SEC23 locus (1 copy reference)

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4BG6

Plasmid

#185877

Purpose

A parental plasmid for in vivo gene amplification (HapAmp) at SEC23 locus

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3AE4

Plasmid

#185873

Purpose

A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (~11 copy)

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3A5C

Plasmid

#185872

Purpose

A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (1 copy reference)

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge