We narrowed to 28,240 results for: INO

1. Tom20-mCh-FKBP

   Plasmid

   #162436

   Purpose

   for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

   Depositor

   Takanari Inoue

   Article

   Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

   Insert

   Tom20-mCh-FKBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28b-pchG WT

   Plasmid

   #87107

   Purpose

   expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

   Depositor

   Audrey Lamb

   Article

   Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

   Insert

   pchG WT
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RP4-6

   Plasmid

   #80385

   Purpose

   Helper plasmid for mobilisation derived from RP4. Spectinomycin/Streptomycin resistant

   Depositor

   Michael Hynes

   Article

   Quandt et al Plasmid. 2004 Jul;52(1):1-12.

   Insert

   Spectinomycin/Streptomycin
   

   Use

   Helper bhr plasmid for mobilizing other plasmids …

   Tags

   none

   Expression

   Mutation

   Promoter

   None

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

4. VPS13D^mScarlet

   Plasmid

   #174110

   Purpose

   The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Codon optimized VPS13D
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   With an internal mScarlet tag in aminoacid positi…

   Promoter

   CMV

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Let-7b-GFP

   Plasmid

   #25404

   Depositor

   Sean Morrison

   Article

   Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

   Insert

   Mirlet7b (Mirlet7b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

6. RSRF-Luc-2wt

   Plasmid

   #31818

   Depositor

   Astar Winoto

   Article

   Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

   Insert

   MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MEF2-dependent (-307 to -242 of Nur77/NR4A1)

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-HA-Uch37

   Plasmid

   #19415

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

   Insert

   Uch37 (UCHL5 Human)
   

   Use

   Tags

   2XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-AncMEcoGII250

   Plasmid

   #205025

   Purpose

   Expressing engineered M.EcoGII with higher thermostability

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 250
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCI-myc-USP10

    Plasmid

    #184034

    Purpose

    Expresses myc-USP10 construct in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Jia et al J Biol Chem. 2021 Jan-Jun;296:100405. do

    Insert

    USP10 (USP10 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMVf

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKm-HCBE

    Plasmid

    #170333

    Purpose

    a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized CBEs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9n-D10A

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKm-HGBE

    Plasmid

    #170334

    Purpose

    a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized GBEs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9n-D10A

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKm-HABE

    Plasmid

    #170332

    Purpose

    a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized ABEs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9n-D10A

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CFP-FKBP

    Plasmid

    #162433

    Purpose

    for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

    Depositor

    Takanari Inoue

    Article

    Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

    Insert

    CFP-FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-HIRA-polyA

    Plasmid

    #59781

    Purpose

    Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    HIRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-HA-Uch37C88A

    Plasmid

    #19416

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    Uch37 (UCHL5 Human)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    C88A

    Promoter

    Available since

    Jan. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-GID1

    Plasmid

    #37306

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GID1
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

18. CF-PIPKi

    Plasmid

    #20158

    Depositor

    Tobias Meyer

    Article

    Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

    Insert

    CFP-FKBP-PIPK (D253A) (Pip5k1c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D253A mutation in PIPK. C-terminus (amino acids …

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRF0G-Tyr

    Plasmid

    #113644

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    MjYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDB038a

    Plasmid

    #99211

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codons at positions 29 and 184 of gIII

    Promoter

    Ppsp and PproK

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEF6 Vps53-V5-6His

    Plasmid

    #175097

    Purpose

    V5 tagged Vps53 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

    Insert

    Vps53 (Vps53 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CF-PIPK

    Plasmid

    #20157

    Depositor

    Tobias Meyer

    Article

    Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

    Insert

    CFP-FKBP-PIPK (Pip5k1c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminus (amino acids 636-661)truncated, R445E,…

    Promoter

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCIG-RARa-403-myc [TJ#344]

    Plasmid

    #16286

    Depositor

    Thomas Jessell

    Article

    Novitch et al Neuron. 2003 Sep 25. 40(1):81-95.

    Insert

    RAR alpha 403 (RARA Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    403 truncation. Dominant repressor derivative.

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pDB038b

    Plasmid

    #99212

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codons at positions 29, 83, and 184 of gIII

    Promoter

    Ppsp and PproK

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCUPI NPD-GFP

    Plasmid

    #1191

    Depositor

    Susan Lindquist

    Article

    Patino et al Science 1996 Aug 2;273(5275):622-6.

    Insert

    N-terminus of Sup35 (SUP35 Budding Yeast)
    

    Use

    Cup1 promoter

    Tags

    GFP

    Expression

    Yeast

    Mutation

    fused N-terminal Sup35 (~1-253aa) to GFP

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

26. pEGFP-C3-Rabaptin-5

    Plasmid

    #199173

    Purpose

    Expresses GFP-Rabaptin-5 fusion in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    Rabaptin-5 (RABEP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains Tyr 149 Ser substitution; insert starts …

    Promoter

    CMV

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEF6 Vps54-V5-6His

    Plasmid

    #175098

    Purpose

    V5 tagged Vps54 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

    Insert

    Vps54 (Vps54 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. VPS13D^Halo

    Plasmid

    #174108

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    With an internal Halo tag in aminoacid position 1…

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NRCoC

    Plasmid

    #74300

    Purpose

    Mammalian expression of full-length treacle fused to GFP

    Depositor

    Rita Shiang

    Article

    Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

    Insert

    treacle ribosome biogenesis factor 1 (Tcof1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDB023f1

    Plasmid

    #99218

    Purpose

    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD4] T7RNAP(S12*,S203*)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TNXXL-CTS20

    Plasmid

    #48342

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    CTS20
    

    Use

    Tags

    TN-XXL

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA4TO OM-eLIR

    Plasmid

    #189004

    Purpose

    mitophagy-inducing adaptor protein

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    OM-eLIR
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSIN ReP5-GFP-GluR1

    Plasmid

    #24004

    Depositor

    Robert Malinow

    Article

    Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

    Insert

    GluR1 (Gria1 Rat)
    

    Use

    Sindbis virus production

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

34. 5HT6-CFP-Venus(H148G)

    Plasmid

    #47501

    Purpose

    Fluorescent reporter for pH inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    Venus(H148G)
    

    Use

    Tags

    5HT6 and CFP

    Expression

    Mammalian

    Mutation

    H148G mutation

    Promoter

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA-Flag-RCC1-polyA

    Plasmid

    #59762

    Purpose

    Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    RCC1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. N-WASP (WGP)

    Plasmid

    #11597

    Depositor

    Marc Kirschner

    Article

    Rohatgi et al J Cell Biol. 2000 Sep 18. 150(6):1299-31

    Insert

    N-WASP (Iqub Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains amino acids 1-396

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

37. pAAV-CMV-iRFP

    Plasmid

    #64887

    Purpose

    Marking retinal neurons with infrared fluorescent proteins (iRFP) to preserve photoresponsiveness

    Depositor

    Tomomi Ichinose

    Article

    Fyk-Kolodziej et al Biotechniques. 2014 Nov 1;57(5):245-53.

    Insert

    iRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHis8-4b-SaDAH

    Plasmid

    #140128

    Purpose

    Expresses SaDAH with N-terminal 8xHis-tag in BL21 E. coli

    Depositor

    Jing-Ke Weng

    Article

    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

    Insert

    Dechloroacutumine halogenase from Sinomenium acutum
    

    Use

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDB023k

    Plasmid

    #99219

    Purpose

    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] T7RNAP(S12*,S203*,S527*)
    

    Use

    Tags

    Expression

    Mutation

    amber codons at positions 12, 203, and 527 of T7 …

    Promoter

    Ppsp

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TNXXL-CTS68

    Plasmid

    #48343

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    CTS68
    

    Use

    Tags

    TN-XXL

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only