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Showing: 1201 - 1240 of 1260 results
  1. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. TRUPATH Triple Ga12

    Plasmid
    #196061
    Purpose
    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. TRUPATH Triple Gai3

    Plasmid
    #196050
    Purpose
    Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlphai3-RLuc8 (GNAI3 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. TRUPATH Triple Ga13

    Plasmid
    #196062
    Purpose
    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. TRUPATH Triple Gagust

    Plasmid
    #196054
    Purpose
    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  13. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. pFBD-BirA_WDR12:90-422

    Plasmid
    #210893
    Purpose
    Insect expression of human WDR12 fragment T90 to G422. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    WDR12:T90-G422 (WDR12 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  15. pFBD-BirA_WDR41:30-459

    Plasmid
    #210892
    Purpose
    Insect expression of human WDR41 fragment Q30 to V459. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    WDR41:Q30-V459 (WDR41 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  16. pFBD-BirA_WDR91:1-747

    Plasmid
    #210879
    Purpose
    Insect expression of full-length human WDR91. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    WDR91:M1-A747 (WDR91 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  17. pFBD-BirA_RACK1:1-317

    Plasmid
    #210889
    Purpose
    Insect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    RACK1:M1-R317 (RACK1 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  18. pFBD-BirA_PRPF4:1-522

    Plasmid
    #210896
    Purpose
    Insect expression of full-length human PRPF4. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    PRPF4:M1-E522 (PRPF4 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  19. pFBD-BirA_CORO1A:1-461

    Plasmid
    #210891
    Purpose
    Insect expression of full-length human CORO1A. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    CORO1A:M1-K461 (CORO1A Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  20. pFBD-BirA_RFWD3:425-774

    Plasmid
    #210884
    Purpose
    Insect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    RFWD3:S425-E774 (RFWD3 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  21. pFBD-BirA_WDR91:392-747

    Plasmid
    #210880
    Purpose
    Insect expression of human WDR91 fragment P392 to A747. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    WDR91:P392-A747 (WDR91 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  22. pFBD-BirA_ATG16L1:303-607

    Plasmid
    #210942
    Purpose
    Insect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    ATG16L1:G303-Y607 (ATG16L1 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 4, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  23. pFBD-BirA_EIF2A:1-427

    Plasmid
    #210938
    Purpose
    Insect expression of human EIF2A fragment M1 to T427. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    EIF2A:M1-T427 (EIF2A Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 4, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  24. pFBD-BirA_COPB2:1-300

    Plasmid
    #210917
    Purpose
    Insect expression of human COPB2 fragment M1 to G300. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    COPB2:M1-G300 (COPB2 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  25. pFBD-BirA_LRRK2:2141-2527

    Plasmid
    #210901
    Purpose
    Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    LRRK2:T2141-E2527 (LRRK2 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    M2397T natural variant ( VAR_024965)
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  26. pFBD-BirA_CDC20:161-P477

    Plasmid
    #210888
    Purpose
    Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    CDC20:S161-P477 (CDC20 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  27. pFBD-BirA_CDC40:236-578

    Plasmid
    #210885
    Purpose
    Insect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    CDC40:E236-W578 (CDC40 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  28. pFBD-BirA_DCAF1:1038-1400

    Plasmid
    #210883
    Purpose
    Insect expression of human DCAF1 fragment E1038 to E1400. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Insert
    DCAF1:E1038-E1400 (DCAF1 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  29. HL2483

    Bacterial Strain
    #62819
    Purpose
    Lim Lab strain: MG1655 + lacIq intergrated at intS + Pcon::TetR inserted into the chromosome at galK + ΔglgCAP + ΔcsrB + ΔcsrC + ΔcsrD
    Depositor
    Bacterial Resistance
    None
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
  30. HL 1255

    Bacterial Strain
    #52941
    Purpose
    MG1655 + fimS (off orientation)::gfp::Asp terminator::CamR at fimA
    Depositor
    Bacterial Resistance
    Chloramphenicol
    Species
    Escherichia coli
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  31. HL 4195

    Bacterial Strain
    #52943
    Purpose
    MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(partial)
    Depositor
    Bacterial Resistance
    Kanamycin
    Species
    Escherichia coli
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  32. HL 6139

    Bacterial Strain
    #52946
    Purpose
    MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(stop codon)
    Depositor
    Bacterial Resistance
    Kanamycin
    Species
    Escherichia coli
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  33. HL 6279

    Bacterial Strain
    #52949
    Purpose
    MG1655 + fimS (off orientation)::gfpAAV tag::Asp terminator::CamR at fimA + ΔlacI + KanR replaces fimB and fimE(partial)
    Depositor
    Bacterial Resistance
    Kanamycin
    Species
    Escherichia coli
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  34. HL 6166

    Bacterial Strain
    #52947
    Purpose
    MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(IRL)
    Depositor
    Bacterial Resistance
    Kanamycin
    Species
    Escherichia coli
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  35. UQ5457

    Bacterial Strain
    #37165
    Depositor
    Bacterial Resistance
    Ampicillin
    Species
    Burkholderia xenovorans
    Available since
    June 21, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 1201 - 1240 of 1260 results