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Plasmid#196055PurposeEncodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorInsertsUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only
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TRUPATH Triple Gai1
Plasmid#196048PurposeEncodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaoA
Plasmid#196051PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensorDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga12
Plasmid#196061PurposeEncodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai3
Plasmid#196050PurposeEncodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga13
Plasmid#196062PurposeEncodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaoB
Plasmid#196052PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensorDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga11
Plasmid#196059PurposeEncodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gagust
Plasmid#196054PurposeEncodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaZ
Plasmid#196053PurposeEncodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga15
Plasmid#196060PurposeEncodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai2
Plasmid#196049PurposeEncodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_WDR12:90-422
Plasmid#210893PurposeInsect expression of human WDR12 fragment T90 to G422. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertWDR12:T90-G422 (WDR12 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_WDR41:30-459
Plasmid#210892PurposeInsect expression of human WDR41 fragment Q30 to V459. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertWDR41:Q30-V459 (WDR41 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_WDR91:1-747
Plasmid#210879PurposeInsect expression of full-length human WDR91. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertWDR91:M1-A747 (WDR91 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_RACK1:1-317
Plasmid#210889PurposeInsect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertRACK1:M1-R317 (RACK1 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_PRPF4:1-522
Plasmid#210896PurposeInsect expression of full-length human PRPF4. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertPRPF4:M1-E522 (PRPF4 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_CORO1A:1-461
Plasmid#210891PurposeInsect expression of full-length human CORO1A. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertCORO1A:M1-K461 (CORO1A Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_RFWD3:425-774
Plasmid#210884PurposeInsect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertRFWD3:S425-E774 (RFWD3 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_WDR91:392-747
Plasmid#210880PurposeInsect expression of human WDR91 fragment P392 to A747. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertWDR91:P392-A747 (WDR91 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_ATG16L1:303-607
Plasmid#210942PurposeInsect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertATG16L1:G303-Y607 (ATG16L1 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 4, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_EIF2A:1-427
Plasmid#210938PurposeInsect expression of human EIF2A fragment M1 to T427. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertEIF2A:M1-T427 (EIF2A Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 4, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_COPB2:1-300
Plasmid#210917PurposeInsect expression of human COPB2 fragment M1 to G300. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertCOPB2:M1-G300 (COPB2 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_LRRK2:2141-2527
Plasmid#210901PurposeInsect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_CDC20:161-P477
Plasmid#210888PurposeInsect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertCDC20:S161-P477 (CDC20 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_CDC40:236-578
Plasmid#210885PurposeInsect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertCDC40:E236-W578 (CDC40 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_DCAF1:1038-1400
Plasmid#210883PurposeInsect expression of human DCAF1 fragment E1038 to E1400. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertDCAF1:E1038-E1400 (DCAF1 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
HL 5226
Bacterial Strain#63667PurposeLim Lab strain: MG1655 + lacIq intergrated at intS + ΔglmZDepositorBacterial ResistanceNoneAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL5410
Bacterial Strain#62820PurposeLim Lab strain: MG1655 + lacIq intergrated at intS + ΔglmZ + DhfqDepositorBacterial ResistanceNoneAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL2483
Bacterial Strain#62819PurposeLim Lab strain: MG1655 + lacIq intergrated at intS + Pcon::TetR inserted into the chromosome at galK + ΔglgCAP + ΔcsrB + ΔcsrC + ΔcsrDDepositorBacterial ResistanceNoneAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 932
Bacterial Strain#52940PurposeMG1655 + ΔlacIDepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 4233
Bacterial Strain#52944PurposeΔfimB + ΔfimE + ΔfimADepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 1285
Bacterial Strain#52942PurposeMG1655 + fimS (off orientation)::gfp::Asp terminator at fimADepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 1255
Bacterial Strain#52941PurposeMG1655 + fimS (off orientation)::gfp::Asp terminator::CamR at fimADepositorBacterial ResistanceChloramphenicolSpeciesEscherichia coliAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 4195
Bacterial Strain#52943PurposeMG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(partial)DepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 6139
Bacterial Strain#52946PurposeMG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(stop codon)DepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 6279
Bacterial Strain#52949PurposeMG1655 + fimS (off orientation)::gfpAAV tag::Asp terminator::CamR at fimA + ΔlacI + KanR replaces fimB and fimE(partial)DepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 6166
Bacterial Strain#52947PurposeMG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(IRL)DepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
UQ5457
Bacterial Strain#37165DepositorBacterial ResistanceAmpicillinSpeciesBurkholderia xenovoransAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only