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KY801: pMVP (L3-L2) P2A-eGFP + polyA

Plasmid

#121764

Purpose

pMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

P2A-eGFP + polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-zebrafish lyn(C466A)–GFP

Plasmid

#35957

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

humanized zebrafish lyn (lyn Zebrafish)

Tags

EGFP

Expression

Mammalian

Mutation

C466A

Promoter

cmv

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S55D

Plasmid

#114032

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 8A/S55D

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP/RFP-miR-E2-4int

Plasmid

#19820

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

Insert

miRNA

Use

Cre/Lox and RNAi

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pIRES2-eGFP-Ofd1-Myc-A80T

Plasmid

#24579

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Alanine 80 changed to threonine (codon GCA to ACA)

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)

Plasmid

#86923

Purpose

To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 1-89 are deleted

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAG415-GPDp-EGFP-rap1-K3NR

Plasmid

#84533

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,117,118)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3CR

Plasmid

#84534

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(462,463,651)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K9R

Plasmid

#84535

Purpose

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K651R

Plasmid

#84536

Purpose

This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K651R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
VV220: CoChR(C108S)-eGFP in fck

Plasmid

#58512

Purpose

Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

CoChR(C108S)-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

changed Cysteine 108 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDZ529 pTRP MET25p PP7-PS-2x-yeGFP

Plasmid

#72233

Purpose

Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

MET25

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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p155 pWZL-EGFP PIP4K delta59N

Plasmid

#11095

Depositor

Ramesh Shivdasani

Insert

PIP4K delta59N

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

delta 59N

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP-Ofd1-Myc-G139S

Plasmid

#24581

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Glycine 139 changed to serine (codon GGT to AGT)

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP-Ofd1-Myc-S437R

Plasmid

#24582

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Serine 437 changed to Arginine (codon AGT to CGT)

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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pDONOR-tagBFP-PSM-EGFP-GBA1

Plasmid

#247978

Purpose

HDR donor vector to revert GBA1 N370S to WT

Depositor

Ellen Sidransky

Article

Chen et al bioRxiv [Preprint]. 2025 Jun 25:2025.06.

Insert

GBA1 Exon 10 to Exon 12 (GBA1 Human)

Use

Hdr donor plasmid

Available Since

June 3, 2026

Availability

Academic Institutions and Nonprofits only

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modRNAc1 L7Ae-eGFP miR142-miR223

Plasmid

#213978

Purpose

L7Ae-P2A-eGFP gene and miR142/223 binding sites in modRNA cap1 vector

Depositor

Xiaoping Bao

Article

Chang et al Nat Biomed Eng. 2026 Apr 24:10.1038/s415

Insert

L7Ae

Use

Synthetic Biology

Tags

eGFP

Expression

Mammalian

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLV EF1a dCas9VP64 2A EGFP 2A BSD

Plasmid

#255458

Purpose

Expresses dCas9VP64, eGFP, and blasticidin

Depositor

Le Cong

Insert

dcas9-vp64

Expression

Mammalian

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-puro-mycProm-EGFP-MYC-U

Plasmid

#255432

Purpose

GFP-tagged MYC with its own 3'UTR

Depositor

Christine Mayr

Inserts

mycProm (MYC Human)
EGFP
MYC plus 3'UTR (MYC Human)

Expression

Mammalian

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pLV_RHOT1 sgRNA_dCas9-KRAB-T2A-EGFP

Plasmid

#253810

Purpose

Express RHOT1 sgRNA under U6 promoter and dCas9-KRAB-T2A-EGFP under hUbC promoter

Depositor

Xinnan Wang

Article

Kwak et al bioRxiv [Preprint]. 2025 Dec 23:2025.12.

Insert

sgRNA RHOT1 (RHOT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

sgRNA for U6p and dCas9-KRAB-T2A-GFP for hUbCp

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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