We narrowed to 10,238 results for: ada

1. pEGPN-ACE2

   Plasmid

   #187483

   Purpose

   Expression vector - ACE2

   Depositor

   Lewis Cantley

   Article

   Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Promoter

   EFS

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SECURE miniABEmax-K20A/R21A (pJUL1774)

   Plasmid

   #131312

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Raichu-OsRac1 CA

   Plasmid

   #133264

   Purpose

   Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(G19V) (LOC4325879 Oryza sativa)
   

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 G19V

   Promoter

   Ubi

   Available Since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Raichu-OsRac1 DN

   Plasmid

   #133263

   Purpose

   Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(T24N) (LOC4325879 Oryza sativa)
   

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 T24N

   Promoter

   Ubi

   Available Since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPR v2-sgAMPK alpha2 clone1

   Plasmid

   #162122

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha2

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-TSC22D3

   Plasmid

   #192916

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   TSC22D3 (TSC22D3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

   Plasmid

   #163545

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-mCherry-N1-iChloC_CA

   Plasmid

   #98171

   Purpose

   slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct iChloC_C128A gene
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E83Q, E90R, E101S, C128A, T159C, D156N

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET-XS-VWF

   Plasmid

   #64847

   Purpose

   Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus

   Depositor

   Sriram Neelamegham

   Article

   Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.

   Insert

   Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Synthetic, Human)
   

   Tags

   7X His, Cerulean, and Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. NC15 pGLUE CUL3

    Plasmid

    #36970

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    CUL3 (CUL3 Human)
    

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pKK-mEGFP-TEV

    Plasmid

    #105777

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mEGFP-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT2-CMV/TetO2-EYFP-GW

    Plasmid

    #153309

    Purpose

    Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Yellow Fluorescent Protein

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDisplay-GRAB_NPYmut

    Plasmid

    #208678

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYpet-Optop38i

    Plasmid

    #89749

    Purpose

    Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i3
    

    Use

    Fluorescent protein

    Tags

    Ypet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJM186A

    Plasmid

    #218127

    Purpose

    Hybrid circuit displaying hsCRBN bait and driving expression of gIII

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    M13 pIII
    

    Tags

    434cI_RR69

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pR26-mBMP7

    Plasmid

    #127374

    Purpose

    Allows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Insert

    BMP7 (Bmp7 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO-GRAB_UCN1.0

    Plasmid

    #208668

    Purpose

    Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBullet-tgn-c

    Plasmid

    #53072

    Purpose

    destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid

    #131137

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid

    #185289

    Purpose

    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    iLID::eGFP::FTH1
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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