We narrowed to 9,747 results for: Ada;
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Plasmid#176248PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2DepositorInserthumanized fnCas12a
UseCRISPR and Synthetic Biology; Expression in micro…TagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_crRNA NR1
Plasmid#176247PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1DepositorInserthumanized fnCas12a
UseCRISPR and Synthetic Biology; Expression in micro…TagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR3
Plasmid#176246PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_crRNA NR3
Plasmid#176249PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3DepositorInserthumanized fnCas12a
UseCRISPR and Synthetic Biology; Expression in micro…TagsExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-MIOX
Plasmid#166680PurposeYeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
wtVP1 + VP2C-GFP
Plasmid#166674PurposeYeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP
Wild-type Murine polyomavirus VP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha2 clone2
Plasmid#162123PurposeLentiviral sgRNA plasmid targeting human AMPK alpha2DepositorInsertsgAMPK alpha2 (PRKAA2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha2 clone3
Plasmid#162124PurposeLentiviral sgRNA plasmid targeting human AMPK alpha2DepositorInsertsgAMPK alpha2 (PRKAA2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A T31A-NG-nCas9
Plasmid#163539PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9
Plasmid#163550PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-HF-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCenpf
Plasmid#160956PurposeCenpf shRNA in pMKO.1 retroviral vectorDepositorInsertCenpf shRNA (Cenpf Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCOrev
Plasmid#153961PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCO
Plasmid#153960PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
S2 1058 ABEMax
Plasmid#135356PurposeExpression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9DepositorInsertnSpyCas9-ABEMax aa 1058
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 CP ABEMax strategy 3
Plasmid#135359PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminusDepositorInsert1029 CP nSpyCas9 ABEMax
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11A
Plasmid#159131PurposeExpresses BACH1 in mammalian cellsDepositorInsertBACH1 (BACH1 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationY11APromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DEPTOR S286/287/291A
Plasmid#159160PurposeExpresses DEPTOR S286/287/291A mutant in mammalian cellsDepositorInsertDEPTOR (DEPTOR Human)
UseTagsHAExpressionMammalianMutationchanged serine 286,287 and 291 to alaninePromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DEPTOR S286/287/291D
Plasmid#159161PurposeExpresses DEPTOR S286/287/291D mutant in mammalian cellsDepositorInsertDEPTOR (DEPTOR Human)
UseTagsHAExpressionMammalianMutationchanged serine 286,287 and 291 to aspartic acidPromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-AAV2-hSyn-MerMAID1-Citrine
Plasmid#126520PurposeAnion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).DepositorInsertMerMAID1
UseAAVTagsCitrineExpressionMutationPromoterhuman synapsinAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S159AS197A
Plasmid#131523PurposeExpresses MKP-3 with S159A mutation and S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Serine 159 to Alanine and Serine 197 to A…PromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only