We narrowed to 17,461 results for: SHE

1. pAAVLINK-EF1alpha-5' PHRF1

   Plasmid

   #236706

   Purpose

   5' AAVLINK plasmid for PHRF1 expression

   Depositor

   Zhonghua Lu

   Insert

   PHRF1 (PHRF1 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   EF1alpha

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG1016-2xBsaI-SaKKH_P2A_EGFP

   Plasmid

   #239461

   Purpose

   Codon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker.

   Depositor

   Alexis Komor

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. p67.uni.pA.OTIIp.mHEL.flag.mCMV.PGK.CD63GFP.WPRE

   Plasmid

   #228765

   Purpose

   mHEL and OT2-peptide

   Depositor

   Ferdinando Pucci

   Insert

   OVA epitope ISQAVHAAHAEINEAGR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQdCas9-sggfp

   Plasmid

   #236184

   Purpose

   The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Quorum sensing promoter

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15bHis6-pG-Tnp

   Plasmid

   #124884

   Purpose

   To transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.

   Depositor

   Keji Zhao

   Insert

   protein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hSyncytin-1(AAAR)

   Plasmid

   #182442

   Purpose

   hSyncytin-1, furin cleavage site RNKR>AAAR(314-317)

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   furin cleacage site RNKR>>AAAR(314-317)

   Promoter

   CMV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG416-GAL-SUP35

   Plasmid

   #181705

   Purpose

   Galactose inducible expression of SUP35

   Depositor

   Alejandro Chavez

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. 0A-1169

   Plasmid

   #220935

   Purpose

   A QUAS line that expresses VSR proteins in Ae. aegypti when crossed with a driver line, Opie-2 dsRed.

   Depositor

   Omar Akbari

   Insert

   Cricket paralysis virus (CrPV) 1A protein, Nodamura virus (NoV) B2 protein, and flock house virus (FHV) B2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-Impact-T4gp32(L301G)

   Plasmid

   #228209

   Purpose

   Expression in BL21(DE3) of single strand DNA binding protein L301G

   Depositor

   Stephen Benkovic

   Insert

   61
   

   Use

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Mutated leucine 301 to glycine (L301G), improves …

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7RNAPNev-FSR22

    Plasmid

    #216338

    Purpose

    Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-SARS-CoV-2 RBD DARPin FSR22

    Depositor

    Mark Styczynski

    Insert

    T7RNAPN-FSR22 fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCG-ACTB-C4

    Plasmid

    #229846

    Purpose

    Expresses wild-type Cas9 and gRNA for ACTB gene.

    Depositor

    Natsuko Chiba

    Insert

    guide RNA for ACTB gene
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCG-NT2

    Plasmid

    #229848

    Purpose

    Expresses wild-type Cas9 and gRNA with non-target sequence.

    Depositor

    Natsuko Chiba

    Insert

    guide RNA with non-target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDual_dsCas9_Venus_hs_NQO1

    Plasmid

    #214684

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human NQO1

    Depositor

    Kevin Janes

    Insert

    dgRNA_NQO1 (NQO1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti puro HA-H2BC21

    Plasmid

    #227589

    Purpose

    Lentiviral plasmid expressing human H2BC21 protein

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    H2BC21 (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CUP1-∆ssCPY*-mScarletI

    Plasmid

    #221157

    Purpose

    Fluorescent reporter for cytoplasmic aggregates

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Use

    Tags

    mScarletI

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CUP1-∆ssCPY*-mNeonGreen

    Plasmid

    #221156

    Purpose

    Fluorescent reporter for cytoplasmic aggregates

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Use

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29b(+)_gp1(A11V)

    Plasmid

    #225131

    Purpose

    Phage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)

    Depositor

    Matej Butala

    Insert

    gp1 (A11V)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Alanine 11 to Valine

    Promoter

    T7 promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIND4-RC-M210TAG-A7

    Plasmid

    #223015

    Purpose

    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for 3-nitrotyrosine

    Depositor

    Steven Boxer

    Inserts

    puhA
    pufL
    pufM
    A7 RS
    pylT tRNA
    

    Use

    Tags

    6xHis

    Expression

    Mutation

    changed Tyr M210 to amber stop codon (TAG) for am…

    Promoter

    rrnB

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIND4-RC-C2-MmXYRS

    Plasmid

    #222744

    Purpose

    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for incorporating haloalkane crosslinker

    Depositor

    Steven Boxer

    Inserts

    puhA
    pufL
    pufM
    MmXYRS
    Mm Pyl tRNA
    

    Use

    Tags

    6xHis

    Expression

    Mutation

    Contains the following substitutions: V346A, W348…

    Promoter

    rrnB

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiP1999 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA

    Plasmid

    #223843

    Purpose

    Enhancer-driven expression of the iCre(R297T) recombinase in thalamic PVT neurons

    Depositor

    Bosiljka Tasic

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Argenine in aa297 was replaced with Threonine

    Promoter

    minBG

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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