We narrowed to 19,563 results for: iNOS

1. PLX306 iCre tetO NICD rtTA

   Plasmid

   #158049

   Purpose

   lenti-viral construct with Cre recombinase and doxycycline inducible expression of mouse Notch intracellular domain

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   mouse Notch Intra Cellular Domain
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   tetO promoter

   Available Since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pM124: pAAV-EFS-CasRx-VEGFA presgRNA

   Plasmid

   #166872

   Purpose

   AAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editing

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Inserts

   U6-VEGFA presgRNA
   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. kiCAP-AAV-M.Mus.2

   Plasmid

   #196682

   Purpose

   Rep/Cap plasmid for the production of M.Mus.2, an AAV capsid with CNS tropism in mice.

   Depositor

   Pardis Sabeti

   Article

   Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   REQQKLW insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-LAMP1-mCherry

   Plasmid

   #214274

   Purpose

   Mammalian expression of LAMP1-mCherry (lysosome marker)

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   LAMP1-mCherry (Lamp1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-NFIB-T2A-miRFP670

   Plasmid

   #187222

   Purpose

   Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Nuclear Factor I B (NFIB Human)
   

   Use

   Tags

   Inserted T2A-miRFP670 at 3' terminal of MCS.

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS1997

   Plasmid

   #49127

   Purpose

   AAV plasmid with SLC6A4 (Ple198) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple198-iCre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SLC6A4

   Available Since

   May 7, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Ana GvpC-SpyTag-His in pET28a

   Plasmid

   #85737

   Purpose

   Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (fused to SpyTag and His-tagged)
   

   Use

   Tags

   His-tag and SpyTag

   Expression

   Bacterial

   Mutation

   Added an extra glycine after the methionine at th…

   Promoter

   T7

   Available Since

   March 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDECKO_mCherry_non-targeting

   Plasmid

   #140109

   Purpose

   CRISPR-Cas9 library validation (negative control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS1943

   Plasmid

   #82565

   Purpose

   High copy number plasmid containing Ple266 (S100B MiniPromoter) insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple266
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pEMS2143

    Plasmid

    #104356

    Purpose

    AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-smCBA-EmGFP-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    smCBA

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL11055

    Plasmid

    #68252

    Purpose

    Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-cGAS R71/75E-HA

    Plasmid

    #130919

    Purpose

    Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    human cGAS R71/75E
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 71 and 75 mutated to glutamate

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. hMLKL-Venus-Mito

    Plasmid

    #106080

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    hMLKL-Venus-Mito (MLKL Human)
    

    Use

    Retroviral; Tet-on retrovirus

    Tags

    mitochondrial-targetting domain of Bcl-xl

    Expression

    Mammalian

    Mutation

    Promoter

    Dox-inducible

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xMma PylT(UUA)_FLAG-AcKRS

    Plasmid

    #140010

    Purpose

    Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    AcKRS-3 (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L301M, L305I, Y306F, L309A, C348F

    Promoter

    EF1

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Igfbp1-promoter/pGL3

    Plasmid

    #12146

    Depositor

    Domenico Accili

    Article

    Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

    Insert

    IGFBP1 promoter (Igfbp1 Rat)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains the canonical insulin response sequence …

    Promoter

    Available Since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pCS2-CRY2-mCherry

    Plasmid

    #140571

    Purpose

    For use in light-induced protein inactivation through the interaction with CIB1

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT5T_hp53_ST

    Plasmid

    #34922

    Depositor

    Thanos Halazonetis

    Article

    Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

    Insert

    p53 DNA-binding (Res 1-393) (TP53 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

    Promoter

    T7

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. BMR1_01g02031-bio-His

    Plasmid

    #108116

    Purpose

    [Edit] Expresses enzymatically monobiotinylated full-length BMR1_01g02031 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag

    Depositor

    Gavin Wright

    Article

    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

    Insert

    BMR1_01g02031
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFL His-mEos3.2-I3

    Plasmid

    #113516

    Purpose

    Expression of His-Eos-I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Use

    Tags

    6*His-mEos3.2

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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