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pRSF-1b-S15-T79C

Plasmid

#133048

Purpose

Expresses tagless S15-T79C mutant ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S15 E.coli ribosomal protein (rpsO E.coli)

Expression

Bacterial

Mutation

T79C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S7-S83C/Δ157-178

Plasmid

#128592

Purpose

Expresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S7 E.coli ribosomal protein (rpsG E.coli)

Expression

Bacterial

Mutation

S83C, Δ157-178

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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p-sfGFP-pA-FRT-Amp-FRT

Plasmid

#176620

Purpose

for generating PCR templates with sfGFP and Amp for BAC recombination

Depositor

Nathan Lawson

Article

Shih et al Development. 2021 Nov 9. pii: 273393. do

Insert

super folder green fluorescent protein with FRT-flanked ampicillin cassette

Use

Unspecified

Mutation

H217R - please see dep. comments

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY282F

Plasmid

#172468

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY282F

Use

Xenopus expression

Tags

none

Mutation

changed Tyrosine at aa282 to Phenylalanine

Promoter

SP6

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlF127P

Plasmid

#174507

Purpose

expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlF127P

Use

Xenopus expression

Tags

none

Mutation

changed Phenylalanine at aa127 to Proline

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlT45A

Plasmid

#172464

Purpose

expresses protein with one aa substitution to affect potential phosphorylation

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlT45A

Use

Xenopus expression

Tags

none

Mutation

changed Threonine at aa45 to Alanine

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY55F

Plasmid

#172466

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY55F

Use

Xenopus expression

Tags

none

Mutation

changed Tyrosine at aa55 to Phenylalanine

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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VTEHDTLLY_epitope

Plasmid

#165017

Purpose

Expression of CMV UL44 (245-253) fused to IL-2 signal sequence

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (245-253)

Use

Lentiviral

Tags

human IL2 signal sequence at N-term

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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NTTVTFLPTPSLILQ_epitope

Plasmid

#165020

Purpose

Expression of CMV UL44 (37-51) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (37-51)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynNanobody IRES EGFP

Plasmid

#136624

Purpose

Plasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-EnhancerNb (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-CMV

Plasmid

#133366

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-CMV-Luc

Plasmid

#133367

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PIR702W-Luc

Plasmid

#133362

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IRFP-702

Tags

HA-tagged IRFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PIR702W

Plasmid

#133361

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IRFP-702

Tags

HA-tagged IRFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-Luc

Plasmid

#133360

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BS

Plasmid

#126203

Purpose

Mammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTR

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIC3Nme

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p1149-pCSDest-AcrIIA4-mCherry-miR122BS

Plasmid

#126205

Purpose

Mammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTR

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIA4

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-MTX_Snifit

Plasmid

#119043

Purpose

Plasmid encoding the bacterial expression of the control Snifit-MTX (FRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

Insert

MTX_Snifit

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R44L H45Q

Promoter

pBAD

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-LUCIDRS-MTX

Plasmid

#113925

Purpose

Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexate

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

LUCID_RS-MTX

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMEsnx5P1gfp

Plasmid

#90131

Purpose

Gateway Mid-entry clone containing promoter sequence specific for snx5 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

snx5P1Egfp (snx5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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