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Showing: 1241 - 1280 of 1648 results

  1. pRH3139

    Plasmid
    #210167
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    R33G
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210167-plasmid-map-sequence-id-416313

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  2. pRH3142

    Plasmid
    #210168
    Purpose
    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1
    Depositor
    Randy Hampton
    Article
    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.
    Insert
    YIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1 (ARO7 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    D147G
    Promoter
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210168-plasmid-map-sequence-id-415820

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  3. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid
    #190174
    Purpose
    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway
    Depositor
    Gary Rudnick
    Article
    Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.
    Insert
    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    Use
    Tags
    FLAG, His6, and c-myc
    Expression
    Mammalian
    Mutation
    changed Cys109 to alanine, changed tyr107 to cyst…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190174-plasmid-map-sequence-id-377202

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  12. pDule2-para-aminoPhe

    Plasmid
    #85503
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85503-plasmid-map-sequence-id-169859

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  14. pBA-mKeima-PIM

    Plasmid
    #174650
    Purpose
    Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.
    Depositor
    Lukas Kapitein
    Article
    Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271
    Insert
    xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    Chicken beta-actin
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    174650-plasmid-map-sequence-id-346673

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  16. pDule-para-aminoPhe

    Plasmid
    #85502
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85502-plasmid-map-sequence-id-169860

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  18. pQCXIP-SHMY-PAUF

    Plasmid
    #182645
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    Pancreatic adenocarcinoma upregulated factor (PAUF)
    Use
    Retroviral
    Tags
    His6-tag, N-terminal ER targeting sequence of hem…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182645-plasmid-map-sequence-id-358618

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  21. pDule2-IBBN (G2)

    Plasmid
    #85501
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85501-plasmid-map-sequence-id-169858

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  25. pET24a-LaM4-3xTS

    Plasmid
    #162777
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162777-plasmid-map-sequence-id-321882

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  27. pYLL204

    Plasmid
    #53455
    Purpose
    Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)
    Depositor
    Lisa Lyu, Leroy Liu
    Article
    Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.
    Insert
    HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Y814F
    Promoter
    CMV
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pDule-IBBN (G2)

    Plasmid
    #85500
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85500-plasmid-map-sequence-id-169863

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  31. pET-30a(+)-Syk-tSH2-PM

    Plasmid
    #111271
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y130E mutation to mimic phosphorylation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111271-plasmid-map-sequence-id-215610

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  32. L-YARS2-9

    Plasmid
    #166533
    Purpose
    scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166533-plasmid-map-sequence-id-329205

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  34. pET24a-LaM4-1xTS

    Plasmid
    #162778
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162778-plasmid-map-sequence-id-321887

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Showing: 1241 - 1280 of 1648 results