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pDmREDusk

Plasmid

#213701

Purpose

Vector for red-light-repressed bacterial gene expression

Depositor

Andreas Moeglich

Article

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHACK-GAL4>QF2

Plasmid

#80275

Purpose

GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pKCyR8

Plasmid

#149494

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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ptac::ccdB

Plasmid

#15922

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

ccdB (ccdB E. coli)

Expression

Bacterial

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2G2

Plasmid

#15791

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pJ2072.2_c1

Plasmid

#140675

Purpose

CRISPRlator control (“open ring”)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

CRISPRlator control (“open ring”)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAd5-B5

Plasmid

#122555

Purpose

Block 5 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 17998-25062

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDrDERusk

Plasmid

#213703

Purpose

Vector for red-light-induced bacterial gene expression

Depositor

Andreas Moeglich

Article

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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hiActTS-GR

Plasmid

#79775

Purpose

high FRET control for ActTS-GR

Depositor

Tetsuya Kitaguchi

Article

Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

Insert

hiActTS-GR

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pPS2991

Plasmid

#8931

Depositor

Pamela Silver

Article

Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-5

Insert

NDR2 (STK38L Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pFLxR3

Plasmid

#167518

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBB530

Plasmid

#27391

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

grpE

Expression

Bacterial

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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piRFP702-N1

Plasmid

#45456

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP702

Expression

Mammalian

Mutation

T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pRMCD28

Plasmid

#167556

Purpose

Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

Type

Empty backbone

Tags

PhoA'

Expression

Bacterial

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIB3

Plasmid

#25452

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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6972 pPROEX-HTb-SSA1

Plasmid

#1231

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Ssa1 (SSA1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
ISA431: aTc-MP6

Plasmid

#189935

Purpose

Mutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanR

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

danQ926, dam, seqA, emrR, ugi and cda1

Expression

Bacterial

Promoter

pTet

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP

Plasmid

#62348

Purpose

An empty optimized gRNA expression vector

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

none

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pFCiR2

Plasmid

#167512

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pJ2018

Plasmid

#140665

Purpose

dCas9, LuxR & Csy4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

dCas9, LuxR & Csy4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP702

Plasmid

#45455

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP702

Expression

Bacterial

Mutation

T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-miRFP704nano

Plasmid

#206190

Purpose

Near-infrared fluorescent protein miRFP704nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDASH-DI-ω1

Plasmid

#242517

Purpose

Donor I vector in DASH and omega1 vector in GoldenBraid/DASH, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDASH-DIS-α2

Plasmid

#242520

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDASH-DII-α1

Plasmid

#242515

Purpose

Donor II vector in DASH and alpha1 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDASH-DII-ω2

Plasmid

#242518

Purpose

Donor II vector in DASH and omega2 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCVA-amiR-PDS

Plasmid

#32911

Depositor

Yule Liu

Article

Tang et al Plant Physiol. 2010 Jun;153(2):632-41. E

Insert

amiR-PDS (Nb)

Use

Plant expression

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2E1

Plasmid

#15787

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E1 (UBE2E1 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-Hisx6-Sirt6

Plasmid

#20277

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
mCherry-FDD

Plasmid

#80629

Purpose

Fluorescent destablizing domain for regulating protein stability and fluorescence

Depositor

Thomas Wandless

Article

Navarro et al ACS Chem Biol. 2016 Jun 6.

Insert

UnaG

Use

Retroviral

Tags

HA-mcherry-linker

Mutation

mutations A36V, R136G

Promoter

LTR

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd5-B2

Plasmid

#122552

Purpose

Block 2 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 3737-10881

Use

Synthetic Biology

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
6920 pPROEXHsp104b

Plasmid

#1230

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (HSP104 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 CIM5 (Rpt-1) GFP

Plasmid

#16074

Depositor

Erin Schuman

Article

Bingol et al Nature. 2006 Jun 29. 441(7097):1144-8.

Insert

CIM5 (RPT1 Budding Yeast)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTG2

Plasmid

#149475

Purpose

Broad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTetA

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416Gal-GFP

Plasmid

#38309

Depositor

Susan Lindquist

Article

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

EGFP (eGFP )

Expression

Yeast

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pDmDERusk

Plasmid

#213702

Purpose

Vector for red-light-induced bacterial gene expression

Depositor

Andreas Moeglich

Article

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
bfloGFPa1

Plasmid

#60749

Purpose

Bacterial expression of Amphioxus GFPa1

Depositor

Dimitri Deheyn

Article

Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

Insert

GFPa1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pFCyR2

Plasmid

#167513

Purpose

Broad-Host range expression vector, RSF1010 origin, CymRAM repressor, PCymRC promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
YCPlac33-sGFP-VRG4

Plasmid

#25447

Depositor

Benjamin Glick

Article

Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

Insert

Synthetic Green Fluorescent Protein - VRG4

Tags

GFP

Expression

Yeast

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI 1+2

Plasmid

#79879

Purpose

Encodes Cas1+2 and a CRISPR array

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1
Cas2
CRISPR Array

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS595 tet-hCMV-EGFPcre

Plasmid

#11957

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

EGFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBLxG2

Plasmid

#149472

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCiB-yin

Plasmid

#13845

Depositor

Ting Wu

Article

Bateman et al Genetics. 2006 Jun . 173(2):769-77.

Insert

yellow (y Fly)

Expression

Insect

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pKCyR6

Plasmid

#149493

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 Hygro EYFP H148Q

Plasmid

#25873

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q mutation

Available Since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only
pBLxVi5

Plasmid

#167516

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, Violacein, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-Violacein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2F

Plasmid

#15800

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2F (UBE2F Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pPEE7

Plasmid

#128357

Purpose

Recycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRG2

Plasmid

#149474

Purpose

Broad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCD185

Plasmid

#113317

Purpose

dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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