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We narrowed to 1,597 results for: tef

Showing: 1551 - 1597 of 1597 results

  14. pcDNA3.1-N-Myc_DED/AAA USP7

    Plasmid
    #131253
    Purpose
    Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131253-plasmid-map-sequence-id-267703

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  16. pRS416-Gal4-dCas9-VP64

    Plasmid
    #71128
    Purpose
    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.
    Depositor
    Ronald Davis
    Article
    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.
    Insert
    Gal4-dCas9-VP64
    Use
    Tags
    NLS n-terminal, N terminal Gal4 Activator domain,…
    Expression
    Yeast
    Mutation
    Promoter
    Tef1
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71128-plasmid-map-sequence-id-127063

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  20. ptdTHC_PGKneoLox2DTA.2

    Plasmid
    #112625
    Purpose
    H2BtdTomato_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox and Mouse Targeting
    Tags
    tdTomato
    Expression
    Mutation
    Promoter
    No Promoter
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112625-plasmid-map-sequence-id-222029

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  21. pCAG-tdTHC

    Plasmid
    #112621
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112621-plasmid-map-sequence-id-222036

    Enlarge

  22. pCAG-VHC

    Plasmid
    #112618
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112618-plasmid-map-sequence-id-222032

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  23. pCAG-mTHC

    Plasmid
    #112620
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112620-plasmid-map-sequence-id-226488

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  24. pVHC_PGKneoLox2DTA.2

    Plasmid
    #112622
    Purpose
    H2BVenus_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Venus
    Expression
    Mutation
    Promoter
    No Promoter
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112622-plasmid-map-sequence-id-222021

    Enlarge

  25. ptdTHC

    Plasmid
    #112617
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112617-plasmid-map-sequence-id-224553

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  26. pVHC

    Plasmid
    #112614
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112614-plasmid-map-sequence-id-224545

    Enlarge

  27. pCAG-EHC

    Plasmid
    #112619
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Emerald (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112619-plasmid-map-sequence-id-226490

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  28. pEHC

    Plasmid
    #112615
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Emerald (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Emerald
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112615-plasmid-map-sequence-id-226350

    Enlarge

  29. pmTHC

    Plasmid
    #112616
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112616-plasmid-map-sequence-id-224548

    Enlarge

  32. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid
    #131255
    Purpose
    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131255-plasmid-map-sequence-id-267709

    Enlarge

  33. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

    Enlarge

  34. pmTHC_PGKneoLox2DTA.2

    Plasmid
    #112624
    Purpose
    H2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox and Mouse Targeting
    Tags
    mTeal (mTFP1)
    Expression
    Mutation
    Promoter
    No Promoter
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112624-plasmid-map-sequence-id-222028

    Enlarge

  35. pEHC_PGKneoLox2DTA.2

    Plasmid
    #112623
    Purpose
    H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Emerald (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Emerald
    Expression
    Mutation
    Promoter
    No Promoter
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112623-plasmid-map-sequence-id-222026

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  38. pcDNA3.1_ZKSCAN1_Tau10-12K317M

    Plasmid
    #194164
    Purpose
    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 WT (MAPT Human)
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Lysine 317 to Methionine
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194164-plasmid-map-sequence-id-381805

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  39. 6-ISG-IFN

    Plasmid
    #231488
    Purpose
    Standard for type I interferon signature (6 genes)
    Depositor
    Stefano Volpi
    Article
    Tesser et al Clin Exp Immunol. 2025 Mar 24:uxaf018. d
    Inserts
    TBP (TBP Human)
    IFI27 (IFI27 Human)
    IFI44L (IFI44L Human)
    IFIT1 (IFIT1 Human)
    ISG15 (ISG15 Human)
    RSAD2 (RSAD2 Human)
    SIGLEC1 (SIGLEC1 Human)
    HPRT1 (HPRT1 Human)
    18s (RPS18 Human)
    G6PD (G6PD Human)
    Use
    Qpcr plasmid standard
    Tags
    Expression
    Mutation
    partial sequence
    Promoter
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231488-plasmid-map-sequence-id-469002

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  41. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9
    Depositor
    Irina Borodina
    Available since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  42. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit
    #1000000236
    Purpose
    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.
    Depositor
    Daniel Voytas
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  43. Cultivarium POSSUM Toolkit

    Plasmid Kit
    #1000000234
    Purpose
    The POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.
    Depositor
    Nili Ostrov
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only

  44. CRISPi Kit

    Plasmid Kit
    #1000000136
    Purpose
    CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.
    Depositor
    Brigitte Gasser
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  45. ScEnSor Kit

    Plasmid Kit
    #1000000215
    Purpose
    This three-step (Build - Transform - Assess) Saccharomyces cerevisiae kit is comprised of two modules (Engineering and Biosensor) that can be used independently or in combination.
    Depositor
    Lisbeth Olsson
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  46. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit
    #1000000138
    Purpose
    Modular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expression
    Depositor
    Elena Caro Bernat
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  47. GoldenPiCS Kit

    Plasmid Kit
    #1000000133
    Purpose
    GoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sites
    Depositor
    Brigitte Gasser
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1551 - 1597 of 1597 results