We narrowed to 3,869 results for: SPL

1. pJYDN

   Plasmid

   #162450

   Purpose

   Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Type

   Empty backbone

   Use

   Shuttle vector bacteria/yeast

   Tags

   Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAXY0007

   Plasmid

   #205576

   Purpose

   NpuDnaE(C)-Glucos(232-500)

   Depositor

   Xiaohan Yang

   Article

   Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

   Insert

   NpuDnaE(C)-Glucosyltransferase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT-Hygro hTERT FL-Firefly

   Plasmid

   #171057

   Purpose

   hTERT Full-length-expressing splicing minigene

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   hTERT FL-Firefly
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC1821_pCR8-SMN2-DN-RG6-SA

   Plasmid

   #118655

   Purpose

   Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   SMN2-DN-RG6-SA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJYDN3

   Plasmid

   #162456

   Purpose

   Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Type

   Empty backbone

   Use

   Shuttle vector bacteria/yeast

   Tags

   ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJYDN2

   Plasmid

   #162454

   Purpose

   Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Type

   Empty backbone

   Use

   Shuttle vector bacteria/yeast

   Tags

   DnbALFA nanobody and Ha and myc

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRegev_D3

   Plasmid

   #204182

   Purpose

   Single reporter with long guanine depleted (G-poor) region from splicing library.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   Exon D3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated CAG

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRegev_D1.61C>G

   Plasmid

   #204179

   Purpose

   pRegev_D1 with single C>G mutation.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   Exon D1 with 61C>G mutation in beta globin splicing minigene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   61C>G mutation

   Promoter

   Truncated CAG

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRegev_D2.30C>G

   Plasmid

   #204181

   Purpose

   pRegev_D2 with single C>G mutation.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   Exon D2 with 30C>G mutation in beta globin splicing minigene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   30C>G mutation

   Promoter

   Truncated CAG

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRegev_D4.34C>G

    Plasmid

    #204185

    Purpose

    pRegev_D4 with single C>G mutation.

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    Exon D4 with 34C>G mutation in beta globin splicing minigene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    34C>G mutation

    Promoter

    Truncated CAG

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRegev_D3.48C>G

    Plasmid

    #204183

    Purpose

    pRegev_D3 with single C>G mutation.

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    Exon D3 with 48C>G mutation in beta globin splicing minigene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    48C>G mutation

    Promoter

    Truncated CAG

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SPELL-GDI Component 2

    Plasmid

    #170487

    Purpose

    SPELL GDI1 C-lobe fused to FRB. Split site is 66

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

    Insert

    FRB-GDI1(C-lobe)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. SPELL-GDI Component 1

    Plasmid

    #170486

    Purpose

    SPELL GDI1 N-lobe fused to iFKBP. Split site is 66.

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

    Insert

    GDI1(N-lobe)-iFKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. plIVMD217

    Plasmid

    #160939

    Purpose

    Excess Capsid Protein in vivo mRNA display

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-mTagBFP2-HA Tag
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. puC19 preACT1(del6) short BP-3'SS

    Plasmid

    #111333

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    shorter branch to 3' splice site distance

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR3.1-OPNc M1

    Plasmid

    #106321

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc mutated splice site EEKQ-->AAAA

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR3.1-OPNc M5

    Plasmid

    #106325

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc splice site insertion mutant EEKQNA-->EEK…

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR3.1-OPNc M4

    Plasmid

    #106324

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc splice site deletion mutant EEKQNA-->EEKNA

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR3.1-OPNc M3

    Plasmid

    #106323

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc mutated splice site EEKQNAV-->AAAAAAA

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSM2

    Plasmid

    #85753

    Purpose

    pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

    Depositor

    Damien Fleetwood

    Article

    Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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