Addgene

We narrowed to 11,657 results for: pcdna

Showing: 621 - 640 of 11657 results

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pcDNA3-HA+WT GADD45

Plasmid

#24929

Depositor

Xin Wang

Article

Yang et al J Biol Chem. 2000 Nov 24. 275(47):36892-

Insert

GADD45 (GADD45A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TOM20_Halo_T2A_EGFP

Plasmid

#135443

Purpose

Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

TOM20-HaloTag7 and EGFP

Use

Tags

TOM20

Expression

Mammalian

Mutation

Promoter

CMV/TetO

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EKAR4

Plasmid

#174437

Purpose

Improved cyan/yellow FRET-based ERK kinase activity reporter.

Depositor

Jin Zhang

Article

Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

Insert

EKAR4

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2

Plasmid

#64932

Purpose

Enhanced FRET-based biosensor for monitoring Akt activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

AktAR2

Use

Tags

Cerulean3 and cpVenus[E172]

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA FoxP3

Plasmid

#229615

Purpose

Mammalian expression of full length mouse FoxP3

Depositor

Sun Hur

Insert

full length FoxP3 (Foxp3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-LZ-CD95L

Plasmid

#104349

Purpose

Expresses human CD95L leucine zipper in mammalian cells

Depositor

Alexander McLellan

Article

Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

Insert

CD95L leucine zipper (FASLG Human)

Use

Tags

octahistidine tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Orange_CaMBI_300

Plasmid

#124095

Purpose

orange calcium-modulated bioluminescent indicator, 300nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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HA-EglN3-pcDNA3

Plasmid

#18960

Depositor

William Kaelin

Article

Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

Insert

Egg laying Nine - 3 (EGLN3 Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-C

Plasmid

#53769

Purpose

mammalian expression of flag-tagged ATR C-terminal fragment

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain, aa 1398-2644*

Promoter

CMV

Available since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-S-Luc_cyto

Plasmid

#113926

Purpose

Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_pMagFast2(3x)-iRFP

Plasmid

#67297

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

pMagFast2(3x)-iRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Beclin1

Plasmid

#21150

Depositor

Junying Yuan

Article

Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

Insert

PcDNA3-Beclin1 (BECN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRPV1exCellHalo

Plasmid

#220307

Purpose

Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

Depositor

Eric Senning

Article

Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

Insert

rTRPV1exCellHalo (Trpv1 Rat)

Use

Tags

circularly permutated Halotag (Deo et al., Nat Ch…

Expression

Mammalian

Mutation

linker included to insert cpHaloTag (

Promoter

CMV promoter

Available since

July 30, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3.1-Nanog

Plasmid

#28221

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Jan 18. ():.

Insert

Nanog (NANOG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3/er-(n2)oxStayGold(c4)

Plasmid

#185822

Purpose

A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

Depositor

Atsushi Miyawaki

Article

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

er-(n2)oxStayGold(c4)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-C-KD

Plasmid

#53770

Purpose

mammalian expression of flag-tagged ATR C-terminal fragment kinase dead mutant

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain, aa 1398-2644, and kinase dead …

Promoter

CMV

Available since

July 8, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA_DENV2-NS2B3_V5

Plasmid

#115906

Purpose

Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

Depositor

Alan Rothman

Article

Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

Insert

DENV2 NS2B3 protease

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Osr1

Plasmid

#26485

Depositor

Paul Danielian

Article

So et al Mech Dev. 1999 Jun . 84(1-2):157-60.

Insert

Osr1 (Osr1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA4-ATR-N

Plasmid

#53767

Purpose

mammalian expression of flag-tagged ATR N-terminal fragment

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

N-terminal domain, aa 1-1409

Promoter

CMV

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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Twitch-2B pcDNA3

Plasmid

#49531

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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