We narrowed to 72,204 results for: plasmid
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Plasmid#145145PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tagDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
Tags8his tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorPublicationInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PT2/C-Luc//PGK-SB13
Plasmid#20207DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFlirefly luciferase/Sb transposase
UseTransposonAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliExpressionBacterialAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKIIa-hChR2(H134R)-mCherry
Plasmid#26975PurposeAAV expression of humanized ChR2(H134R) fused to mCherry driven by CaMKIIa promoter for optogenetic excitationDepositorHas serviceAAV1, AAV5, AAV9, and AAV RetrogradeInserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterCaMKIIaAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pB-CAGGS-dCas9-KRAB-MeCP2
Plasmid#110824PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-KRAB-MeCP2
ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSQT1601
Plasmid#53369Purposehuman codon optimized Csy4 and FokI-dCas9 expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthCsy4-T2A-NLS-hFokI-dCas9-NLS
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDawn
Plasmid#43796PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2FLAG
Plasmid#16331DepositorInsertFlag tag
UseVertebrate expressionTagsFlagMutationFlag tag inserted into pCS2+ polylinkerAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAKlux2
Plasmid#14080DepositorInsertBacterial luciferase
ExpressionBacterialPromoterLac, T3Available sinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
8xGTIIC-luciferase
Plasmid#34615PurposeYAP/TAZ-responsive synthetic promoter driving luciferase expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsynthetic TEAD luciferase reporter
UseLuciferaseAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
T4 Lysozyme WT*
Plasmid#18111DepositorAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLENTI_hACE2_PURO
Plasmid#155295PurposeLentiviral vector to generate hACE2 stable expressing cell lineDepositorAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
FH-TET1-pEF
Plasmid#49792PurposeThis plasmid expresses the full-length human TET1 gene with a Flag and HA tag.DepositorInsertTet methylcytosine dioxygenase 1 (TET1 Human)
TagsFlag and HAExpressionMammalianPromoterEF-1alphaAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV5 mouse src
Plasmid#13663DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsrc (Src Mouse)
TagsMyrExpressionMammalianPromoterCMVAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-dCas9-KRAB-blast
Plasmid#89567PurposePlasmid expression dCas9 protein in fusion with KRAB domainDepositorInsertdCas9
UseLentiviralTagsBlasticidin resistance gene and KRABMutationD10A and H840AAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSQT1601
Plasmid#53369Purposehuman codon optimized Csy4 and FokI-dCas9 expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthCsy4-T2A-NLS-hFokI-dCas9-NLS
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-Cre-P2A-dTomato (AAV Retrograde)
Viral prep#107738-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only