We narrowed to 18,318 results for: Mut

1. pmCherry-Synaptojanin-1-145

   Plasmid

   #223563

   Purpose

   Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

   Depositor

   Pietro De Camilli

   Article

   Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   SYNJ1 (SYNJ1 Human)
   

   Use

   Tags

   His and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGGD_MiniTurboID-YFP

   Plasmid

   #222434

   Purpose

   Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.

   Depositor

   Dominique Bergmann

   Article

   Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

   Insert

   MiniTurboID (BirA mutant)
   

   Use

   Golden gate / green gate compatible cloning vector

   Tags

   Linker and YFP

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICE-HA-MRE11-H129N

   Plasmid

   #82034

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Nuclease-dead mutant: H129N MRE11

   Promoter

   CMV-tet

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Neo-Bam APC 1-1941

   Plasmid

   #16510

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon 1941 truncation

   Promoter

   Available Since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

   Plasmid

   #158962

   Purpose

   Cre-dependent Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   hsp70l promoter
   floxed DsRed cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Neo-Bam APC 1-2644

   Plasmid

   #16511

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon 2644 truncation

   Promoter

   Available Since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICE-HA-MRE11-H63D

   Plasmid

   #82036

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Exonuclease-dead mutant: H63D MRE11

   Promoter

   CMV-tet

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28a(+)-FGF1-4X

   Plasmid

   #120282

   Purpose

   Produce human recombinant FGF1-4X in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Basic fibroblast growth factor 1 mutant
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF155 pcDNA3.1(+)SOD1G93A

   Plasmid

   #26401

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G93A

   Promoter

   Available Since

   Nov. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG491

    Plasmid

    #226747

    Purpose

    CRISPRi guide RNA against MTCH2 (dual guide)

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MTCH2 (MTCH2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG43

    Plasmid

    #226736

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MICU1 (targeting sequence) (MICU1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Neo-Bam APC 1-331

    Plasmid

    #16508

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    APC (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon 331 truncation

    Promoter

    Available Since

    April 30, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-CXCL12-sfGFP

    Plasmid

    #98961

    Purpose

    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCL12 (CXCL12 Human)
    

    Use

    Tags

    Superfolder GFP

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGW03 AAV-TBG-Cre-sgRNA

    Plasmid

    #192163

    Purpose

    AAV-TBG-Cre-sgRNA

    Depositor

    Sidi Chen

    Article

    Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. R4pGWB601_UBQ10p-Turbo-NES-YFP

    Plasmid

    #127366

    Purpose

    Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH

    Plasmid

    #184463

    Purpose

    pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH express N-flag tag STAT5bCA carrying a point mutation at base 1963 (A to C), which changes Asn642 to His, conferring constitutive activity (CA) to STAT5b.

    Depositor

    Rhonda Kineman

    Article

    Lau-Corona et al Endocrinology. 2022 Apr 9. pii: 6565757.

    Insert

    N-Flag-mSTAT5bCA (Stat5b Mouse)
    

    Use

    AAV

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Point mutation at base 1963 (A to C), which chang…

    Promoter

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUG-Pten

    Plasmid

    #140732

    Purpose

    Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-Pten (PTEN Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hUbiC

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. AB-GFP

    Plasmid

    #72203

    Purpose

    screen AB42 variants for aggregation

    Depositor

    Michael Hecht

    Article

    Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

    Insert

    AB42 (APP Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-ExRai-AKAR2(T/A)

    Plasmid

    #161754

    Purpose

    Negative-control mutant for ExRai-AKAR2 biosensor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2(T/A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-beta-catenin-S33Y

    Plasmid

    #13371

    Purpose

    Retroviral expression of mouse beta-catenin S33Y mutant

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    catenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Serine 33 to Tyrosine and Leucine 388 to Proline

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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