We narrowed to 19,557 results for: Mut
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pICE-HA-MRE11-H63S
Plasmid#82035PurposePlasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(D323A/D325A)
Plasmid#86592PurposeExpresses human full-length Akt1(D323A/D325A) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
U6-pegRNA-H1-nick sgRNA-mCherry
Plasmid#176901PurposeExpresses prime editing guide RNA and guide RNA in mammalian cellsDepositorInsertsmCherry
gRNA scaffold
gRNA scaffold
UseAAVExpressionMammalianPromoterCAG, H1, and U6Available SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-Sumo1 Q94P
Plasmid#48965PurposeExpresses HA SUMO1 Q94P mutant in mammalian cellsDepositorAvailable SinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFPLaminA-N195K
Plasmid#32708DepositorAvailable SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Tlr4
Plasmid#27148DepositorInserttoll-like receptor 4 (Tlr4 Mouse)
Tags3x FLAGExpressionMammalianMutationNative signal peptide sequence (original amino ac…Available SinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMJ923
Plasmid#78313PurposeExpression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-mCherry-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYDE009
Plasmid#194151PurposeDelivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.DepositorInserttetR-tetP-dcas9-rrnB T1-T7Te
ExpressionBacterialPromotertetpAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shRFP
Plasmid#110940PurposeTet-inducible shRNA targeting RFPDepositorInsertshRFP
UseLentiviral and RNAiPromoterH1/TOAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR3c-WT
Plasmid#45711DepositorAvailable SinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFPLaminA-L85R
Plasmid#32707DepositorAvailable SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg6_I
Plasmid#146551PurposeMammalian Expression of HsSmg6DepositorInsertHsSmg6 (SMG6 Human)
ExpressionMammalianMutationtwo silent mutations T594C, A699G and two non sil…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-Ns3h-UGI
Plasmid#221219PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase.DepositorInsertAID-Ns3h-UGI
ExpressionMammalianAvailable SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-nSpCas9
Plasmid#171691PurposeExpresses nSpCas9 in mammalian cellsDepositorInsertnSpCas9
UseCRISPRExpressionMammalianMutationD10APromoterEF1aAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DU422gp140-TM
Plasmid#123251PurposeMammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tetherDepositorInsertHIV-1 (DU422) Env
TagsCD5 leader peptide and Linker (with 6his tag) and…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-TBP6.9F34MF37MF77M
Plasmid#168268PurposeExpresses human TBP6.9 with the F34M/F37M/F77M triple mutantDepositorInsertTBP6.9
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F34M, F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-CCSS
Plasmid#83424PurposeMouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
TagsEGFPExpressionMammalianMutationResidues 218 and 219 from C to S (CCSS: TGCTGT -&…PromoterCMVAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC-HYG-YR
Plasmid#61765PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).DepositorInsertsHygromycin
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…PromotertrpC promoterAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
Tags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only
