We narrowed to 12,154 results for: NSI;

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pDEST- IP2.0(codon for C.elegans)

Plasmid

#109216

Purpose

IP2.0 for C. elegans (Codon optimization and introns).

Depositor

Takeshi Ishihara

Article

Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

Insert

IP2.0 (Inverse Pericam 2.0)

Tags

His-tag

Expression

Worm

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

Plasmid

#101298

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-698 of alpha-Catenin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBeast-pAhpC-RBS- LacZ

Plasmid

#190060

Purpose

LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

lacZ

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMTTH+H1.4ΔCTD

Plasmid

#157797

Purpose

Expression of human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4ΔCTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal G294A YFP

Plasmid

#27448

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191155

Purpose

Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:DNAJB1.s1:EGFP-3xMS2

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry

Plasmid

#75132

Purpose

Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
pGEX6P1-Ubl

Plasmid

#78712

Purpose

Expresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 2-85

Tags

GST-Tag

Expression

Bacterial

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_TAOK2

Plasmid

#172979

Purpose

doxycycline-inducible expression of TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

TAOK2 (TAOK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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All-in-one split ADAR2(E488Q)

Plasmid

#170137

Purpose

AAV vector carrying ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_ARP2

Plasmid

#111658

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

ARP2 (ACTR2 Human)

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS3

Plasmid

#104353

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Expression

Mammalian

Mutation

AA1-733 ITGB2 present

Promoter

CMV

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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LifeAct-mAvicFP1

Plasmid

#129512

Purpose

Expresses LifeAct-mAvicFP1 in mammalian cells

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

LifeAct-mAvicFP1

Expression

Mammalian

Mutation

mAvicFP1 is AvicFP1 F64L + A206K

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP-shACTN3

Plasmid

#52678

Purpose

shRNA against α-actinin-3 with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Insert

ACTN3 (Actn3 Rat)

Use

RNAi

Tags

CMV-EGFP

Promoter

U6

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgKEAP1-2

Plasmid

#74986

Purpose

sgKEAP1-2, puromycin selection

Depositor

William Hahn

Article

Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

Insert

KEAP1

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCold TDP43 G294A

Plasmid

#27452

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa and His6

Expression

Bacterial

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pHH0103_UBASH3B-1/1

Plasmid

#91462

Purpose

Protein expression and purification of human SH3 domain construct UBASH3B-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

UBASH3B-1/1 (UBASH3B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBoBi-hALDOA

Plasmid

#210806

Purpose

For stably expression in cells

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pUBQ10:AtFLS2

Plasmid

#202186

Purpose

Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

Depositor

Gitta Coaker

Article

Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

Insert

Flagellin sensing 2 (FLS2 Mustard Weed)

Tags

HA

Expression

Plant

Promoter

AtUBQ10

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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