We narrowed to 40,158 results for: KAN;

1. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

   Plasmid

   #216250

   Purpose

   HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human SEC61B (SEC61B HDR template, Synthetic, Human)
   

   Use

   CRISPR; Hdr template

   Tags

   BSD.P2A.miniIAA7.3xFlag

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a_Puro_Telo_v2

   Plasmid

   #195139

   Purpose

   ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Telomere (RTEL1 Human)
   

   Expression

   Mammalian

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSSRB274_pAC8-eGFP-hsCRBN

   Plasmid

   #218791

   Purpose

   Insect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBN

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   Protein cereblon (CRBN Human)
   

   Tags

   FLAG-TEV-eGFP-Prescission

   Expression

   Insect

   Mutation

   eGFP fused to N-terminus

   Promoter

   polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-45b-POLR2A-CTD-mCherry

   Plasmid

   #185011

   Purpose

   Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   POLR2A (Polr2a Mouse)
   

   Expression

   Bacterial

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCVpuro-Mito-Pericam

   Plasmid

   #87381

   Purpose

   Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.

   Depositor

   Björn Stork

   Article

   Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

   Insert

   Mito-Pericam [ratiometric]
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Retroviral 5' LTR promoter

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-MCS-MED1long-IDR-YFP-NLS

   Plasmid

   #145276

   Purpose

   Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MED1-IDR long (MED1 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA

   Plasmid

   #113160

   Purpose

   A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR4_miniFXN_7

   Plasmid

   #234360

   Purpose

   Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.

   Depositor

   Marek Napierala

   Article

   Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

   Insert

   FXN (FXN Human)
   

   Use

   Cloning

   Tags

   Flag Tag

   Mutation

   There is a partial deletion in the 5'UTR of …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-MEKDD

   Plasmid

   #31202

   Purpose

   Gateway entry vector containing MEKDD.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   MAPKK1 (MAP2K1 Human)
   

   Use

   Gateway donor vector

   Mutation

   S218D S222D

   Available Since

   June 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a_Puro_Telo_v1

    Plasmid

    #195138

    Purpose

    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Telomere (RTEL1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-Tet-On-PLXNB2-shRNA1

    Plasmid

    #98399

    Purpose

    Lentivirus for expression of shRNA1 against human PLEXIN-B2 (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX458_mCherry-EWSR1-gRNA

    Plasmid

    #237683

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to EWSR1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EWSR1 gRNA (EWSR1 Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5_mEGFP-SS18-SSX2

    Plasmid

    #237637

    Purpose

    For overexpression of mEGFP-SS18-SSX2

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    SS18-SSX2 (SS18, SSX2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYX233-b2-DHFR

    Plasmid

    #163759

    Purpose

    Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    b2-DHFR (Dhfr Mouse, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

    Promoter

    GAL1

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS

    Plasmid

    #145270

    Purpose

    Mammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBP(53Q)-IDR (TBP Human)
    

    Expression

    Mammalian

    Mutation

    Q-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS

    Plasmid

    #145269

    Purpose

    Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBP(38Q)-IDR (TBP Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 61426-hU6-shmFmr1-EF1a-mCherry

    Plasmid

    #157859

    Purpose

    Expresses a shRNA targeting mouse Fmr1 gene

    Depositor

    Xinyu Zhao

    Article

    Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

    Insert

    Fmr1 (Fmr1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. cryS96

    Plasmid

    #67519

    Purpose

    αB-crystallin minipeptide fused to ELP (S96).

    Depositor

    Andrew MacKay

    Article

    Wang et al J Control Release. 2014 Oct 10;191:4-14.

    Insert

    mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)
    

    Tags

    elastin-like polypeptide (ELP) S96

    Expression

    Bacterial

    Mutation

    mini-αB-crystallin contains residues 73–92 of aB-…

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. retro-gfp-puro vector

    Plasmid

    #58249

    Purpose

    Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    EGFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. GLDC-RES

    Plasmid

    #72882

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    GLDC (GLDC Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    silent mutations in site corresponding to shGLDC_2

    Promoter

    CMV

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only