We narrowed to 17,078 results for: Por

1. pSI-535

   Plasmid

   #131130

   Purpose

   gRNA expression vector for C-to-T base editing reporter

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   C-to-T reporter activation gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-PKCsub-YPet-NES

   Plasmid

   #138207

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKCsub-YPet-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Oct4-Cr1

   Plasmid

   #66989

   Purpose

   Expresses gRNA that targets stop codon for OCT4

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

   Insert

   gRNA for Crispr targeting the OCT4 stop codon
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pBICD2-TUG

   Plasmid

   #52873

   Purpose

   retroviral expression of TUG

   Depositor

   Jonathan Bogan

   Article

   Bogan et al Nature. 2003 Oct 16;425(6959):727-33.

   Insert

   TUG (Aspscr1 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-CD2 (mouse)

   Expression

   Mammalian

   Mutation

   Promoter

   retrovirus LTR

   Available Since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Tac-GFP

   Plasmid

   #162494

   Purpose

   Expresses Tac (IL2 recptor alpha subunit) with C-terminally tagged GFP in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   Tac (IL2RA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. plko.1/shMCT4 477

   Plasmid

   #99598

   Purpose

   Knockdown of MCT4 expression

   Depositor

   Eli Bar

   Article

   Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

   Insert

   MCT4 (SLC16A3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAL190

   Plasmid

   #22279

   Purpose

   Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-bArrestin2-TevC-P2A-TdTomato

   Plasmid

   #89869

   Purpose

   expresses iTango bArrestin2-TevC component and TdTomato bicistronically

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   bArrestin2-TevC-P2A-TdTomato
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   R173H in TdTomato (please see depositor comments …

   Promoter

   CMV

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHA-ΔN335-Kin1A

   Plasmid

   #31606

   Depositor

   Anthony Brown

   Article

   Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

   Insert

   ΔN335 kinesin-1A (Kif5a Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-335

   Promoter

   CMV

   Available Since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

10. TXNIP-py

    Plasmid

    #200090

    Purpose

    Dox-inducible human TXNIP with py motif mutations (ppcy,ppty -->paca,pata) in pInducer20 vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    TXNIP (TXNIP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    py motifs mutated (aa331-334 and 375-378)

    Promoter

    CMV

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Tac

    Plasmid

    #162489

    Purpose

    Expresses Tac (IL2 recptor alpha subunit) with GFP tag in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    Tac (IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDN43

    Plasmid

    #61346

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS11 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLexm-gfp-Pept2

    Plasmid

    #167988

    Purpose

    expresses rat PepT2 with a c terminal cleavable GFP-His tag.

    Depositor

    Simon Newstead

    Article

    Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

    Insert

    PepT2 (Slc15a2 Rat)
    

    Use

    Tags

    cleavable GFP-his8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMPMA3dPlac-PsopB-GFPLVA

    Plasmid

    #23341

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Use

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs_FLAG-SFXN3

    Plasmid

    #110637

    Purpose

    Retroviral expression of Flag-SFXN3 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN3 (SFXN3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

    Plasmid

    #108375

    Purpose

    FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGREAT18

    Plasmid

    #170906

    Purpose

    GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pAtUBQ10

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEasyG1_zeo

    Plasmid

    #184910

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMIII-Neo-tdTomato

    Plasmid

    #153424

    Purpose

    Retroviral (MSCV) expression of tdTomato fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    tdTomato
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRX-IP/SECFP-mDFCP1

    Plasmid

    #58765

    Purpose

    Expresses SECFP-mDFCP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    SECFP

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only