We narrowed to 42,746 results for: TRO

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pTRE3G-orangutan cGASdeltaN-GFP

Plasmid

#186846

Purpose

Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal GFP tag (CGAS Pongo abelii, Synthetic)

Use

Retroviral

Tags

GFP

Mutation

N-terminal truncation

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_hnRNPA1_allW

Plasmid

#224252

Purpose

Bacterial expression of N-terminally 6His-tagged hnRNPA1_allW

Depositor

Tanja Mittag

Article

Alshareedah et al Nat Phys. 2024 Sep;20(9):1482-1491. doi:

Insert

hnRNPA1_allW (HNRNPA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

F17W,F25W,F31W,F37W,F43W,Y52W, Y59W, F62W, F69W, …

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-ChloC

Plasmid

#179841

Purpose

donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

chloride-conducting ChR (ChloC)

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-iChloC

Plasmid

#179842

Purpose

donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

improved chloride-conducting ChR (iChloC)

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-luciferase

Plasmid

#179844

Purpose

donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Tags

luciferase

Expression

Mammalian

Promoter

Troponin T promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-POLB

Plasmid

#215138

Purpose

Expresses mCherry-POLB in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

POLB (POLB Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgC0111-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209062

Purpose

Entry vector that encodes sgRNAs against mouse Rb1, Trp53, Rbl2, and a non-targeting sgRNA (C0111) and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

Insert

non-targeting sgRNA (C0111)

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.3

Plasmid

#206137

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx26G45E-IRES-GCaMP6s

Plasmid

#188237

Purpose

3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 variant p.G45E and GCaMP6s

Depositor

Fabio Mammano

Article

Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

Insert

GJB2 (GJB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

p.G45E

Promoter

Tight TRE promoter

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-R/G-ICUE

Plasmid

#181849

Purpose

ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

R/G-ICUE (RAPGEF3 Human)

Tags

mRuby2 and sfGFP

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG membrane-bound lipin-1 (delta200)

Plasmid

#120278

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the delta200 Golgi-…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_PSG2_WT

Plasmid

#81917

Purpose

Gateway Donor vector containing PSG2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PSG2 (PSG2 Human)

Use

Gateway entry vector

Mutation

V20L; p.333_335del-NPT-ins-IQH

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMR3A_WT

Plasmid

#81887

Purpose

Gateway Donor vector containing SMR3A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMR3A (SMR3A Human)

Use

Gateway entry vector

Mutation

C42R; P132L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_C1orf150_WT

Plasmid

#81842

Purpose

Gateway Donor vector containing C1ORF150 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C1ORF150 (GCSAML Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPAG8_WT

Plasmid

#81848

Purpose

Gateway Donor vector containing SPAG8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPAG8 (SPAG8 Human)

Use

Gateway entry vector

Mutation

E2D; E5G; P226L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CPXCR1_WT

Plasmid

#81819

Purpose

Gateway Donor vector containing CPXCR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CPXCR1 (CPXCR1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2M2_WT

Plasmid

#81758

Purpose

Gateway Donor vector containing OR2M2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

R220G, C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MTM1_p.R253Q

Plasmid

#81724

Purpose

Gateway Donor vector containing MTM1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Mutation

R253Q; E410K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MTM1_p.P226S

Plasmid

#81678

Purpose

Gateway Donor vector containing MTM1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Mutation

P226S; E410K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_OR2M2_p.N42Y

Plasmid

#81638

Purpose

Gateway Donor vector containing OR2M2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

OR2M2 (OR2M2 Human)

Use

Gateway entry vector

Mutation

N42Y; R220G; C235R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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