We narrowed to 18,318 results for: Mut

1. pCMV-hAR-W433A,L436A,F437A

   Plasmid

   #89086

   Purpose

   mammalian expression of human androgen receptor with mutations of N-terminal WxxLF motif

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   hAR WxxLF mutant (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR-W433A,L436A,F437A

   Promoter

   CMV

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL Blast Twist ER

   Plasmid

   #18799

   Depositor

   Bob Weinberg

   Article

   Mani et al Cell. 2008 May 16. 133(4):704-15.

   Insert

   twist (Twist1 Mouse)
   

   Use

   Retroviral

   Tags

   ER hormone binding domain

   Expression

   Mammalian

   Mutation

   G525R mutation in the ER. The presence of the G52…

   Promoter

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-DNM2-R465W

   Plasmid

   #155262

   Purpose

   Mammalian expression of rat DNM2 fused to HA

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Arginine 465 to Tryptophan

   Promoter

   Available Since

   May 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-ATF6-(S1P-)

   Plasmid

   #32956

   Purpose

   For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.

   Depositor

   Ron Prywes

   Article

   Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

   Insert

   ATF6 (ATF6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S1Protease site mutation: R415A/R416A

   Promoter

   CMV

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCPH2A.X_S139A-GS3

   Plasmid

   #204749

   Purpose

   Expression of Cas9 and human H2AX with the S139A mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX S139A (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the S139A mutation

   Promoter

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SOX9-T2A-H2B-EGFP repair template

   Plasmid

   #167972

   Purpose

   SOX9 reporter repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 9 (SOX9 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LUC01 (L/Sec/P)

   Plasmid

   #112144

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U)

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

   Plasmid

   #127355

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMVS102_P3-GFP-NATMX6

   Plasmid

   #99048

   Purpose

   EGFP reporter with six binding sites for the Zif268 DNA binding domain

   Depositor

   Barak Cohen

   Article

   Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

   Inserts

   McIsaac 2014 P3 promoter
   eGFP
   clonNAT resistance
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL1 promoter with 4 GAL4 sites removed and repla…

   Promoter

   McIsaac 2014 P3 promoter

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-BAF_L58R

    Plasmid

    #101776

    Purpose

    Expresses EGFP tagged mutant BAF (L58R) in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L58R mutation

    Promoter

    EF1a

    Available Since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4-ATR-C-KD

    Plasmid

    #53770

    Purpose

    mammalian expression of flag-tagged ATR C-terminal fragment kinase dead mutant

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal domain, aa 1398-2644, and kinase dead …

    Promoter

    CMV

    Available Since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pC-FLAG-ZCD2

    Plasmid

    #79274

    Purpose

    Mammalian expression of ZCD2 with a C-terminal FLAG tag

    Depositor

    Rita Shiang

    Article

    Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

    Insert

    ZCD2 (CISD2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid

    #127356

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-Sec24D

    Plasmid

    #32678

    Depositor

    Henry Lester

    Article

    Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9.

    Insert

    Sec24D (SEC24D Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. LAMP1-mCherry-FRB*

    Plasmid

    #207145

    Purpose

    Lysosome-targeted rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    FRB* (T2098L mutant of FKBP-rapamycin binding dom…

    Expression

    Mammalian

    Mutation

    Silent mutation

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBpuro-EGFR(M721)

    Plasmid

    #197379

    Purpose

    Encoding human ErbB1 mutant.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    EGFR mutant (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lysine 721 to Methionine

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBp-FGFR2c-WT

    Plasmid

    #45699

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-HA-Akt1-K179M

    Plasmid

    #73409

    Purpose

    Expresses kinase-inactive mutant of Akt with HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed K179M to make Kinase inactive mutant

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mcherry p62 delta UBA silent

    Plasmid

    #187984

    Purpose

    mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lacking UBA domain (aa389-434) but it has the sil…

    Promoter

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-CXCR4

    Plasmid

    #98944

    Purpose

    Mammalian expression plasmid for untagged human CXCR4

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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