We narrowed to 31,812 results for: LIS;

1. pET21-pelB-LaMIgG-14

   Plasmid

   #228994

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-14
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaTdT-39

   Plasmid

   #228980

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-39
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-3-FT-ENGRAM

   Plasmid

   #179158

   Purpose

   piggyBac plasmid for 3'-FT ENGRAM recorder

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Insert

   Csy4
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   minP

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYLsgRNA-AtU3d

   Plasmid

   #66200

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   AtU3d

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-DsRed2

   Plasmid

   #112219

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   DsRed2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   DsRed2 contains a silent nucleotide substitution …

   Available Since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCardinal-ZO1-C-14

   Plasmid

   #56179

   Purpose

   Localization: Tight Junctions, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   ZO1 (TJP1 Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCAG_mRuby2_smFP OLLAS

   Plasmid

   #59761

   Purpose

   encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   mRuby2_smFP_OLLAS
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-RAB35

   Plasmid

   #164630

   Purpose

   Expresses RAB35 in mammalian cells with a GFP tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   RAB35 (RAB35 Human)
   

   Tags

   cycle 3 GFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MiniCoopR mitfa:KIT K642E

   Plasmid

   #118849

   Purpose

   Expresses human KIT K642E mutant and zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   KIT K642E (KIT Human, Zebrafish)
   

   Use

   Zebrafish expression, tol5

   Mutation

   KIT K642E

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. UbC-Halo-SFPQ

    Plasmid

    #166946

    Purpose

    Lentiviral plasmid expressing Halo-tagged SFPQ protein from the UbC promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    Halo

    Promoter

    UbC

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA pDEST40 Cas9-POLD3

    Plasmid

    #183194

    Purpose

    Expression clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    POLD3
    

    Use

    CRISPR

    Tags

    Cas9WT

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40

    Plasmid

    #233365

    Purpose

    CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    Kinprola_PKA-mEGFP-NLS3X
    

    Use

    AAV

    Tags

    mEGFP-NLS3X

    Promoter

    CAG

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_CAG_NES-Kinprola_PKA-mTagBFP2_WPRE-SV40

    Plasmid

    #233364

    Purpose

    CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mTagBFP2 for mammalian cell expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_PKA-mTagBFP2
    

    Use

    AAV

    Tags

    NES and mTagBFP2

    Promoter

    CAG

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-26b-NbVHH05-HA-His

    Plasmid

    #171567

    Purpose

    Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-HA-His (UBE2J1 Human)
    

    Tags

    HA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rb-379-792-pGex2TK

    Plasmid

    #21748

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

    Insert

    Rb (RB1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-792 of wt Rb …

    Available Since

    Aug. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUW-TetO-Irf7

    Plasmid

    #178445

    Purpose

    doxycycline-inducible expression of Mouse Irf7 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Irf7 (Irf7 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. IR25a-T2A-QF-AD

    Plasmid

    #162526

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
    

    Expression

    Insect

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-hA3A-eBE-Y130F

    Plasmid

    #113423

    Purpose

    Expresses hA3A-eBE-Y130F in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-eBE-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_Y130F

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-Cepheid1s-ST-WPRE

    Plasmid

    #214968

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostability

    Depositor

    Peng Zou

    Article

    Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

    Insert

    Cepheid1s-ST
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTag-BFP-C-h-Rab11a

    Plasmid

    #79805

    Purpose

    Express TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab11a (RAB11A Human)
    

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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