We narrowed to 6,120 results for: nls

1. pJM589 EF1α intN-ZF10-NLS-HA in TUPV2

   Plasmid

   #161565

   Purpose

   Constitutive expression of intN-ZF10-NLS-HA under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   intN-ZF10-NLS-HA
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJB004 CMV 3xFLAG-NLS-VP16-intN

   Plasmid

   #161549

   Purpose

   Constitutive expression of 3xFLAG-NLS-VP16-intN under the CMV promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-VP16-intN
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJB005 CMV 3xFLAG-NLS-intC-ZF2

   Plasmid

   #161550

   Purpose

   Constitutive expression of 3xFLAG-NLS-intC-ZF2 under the CMV promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-intC-ZF2
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJM516 EF1α intC-ZF1-NLS-HA in TUPV2

   Plasmid

   #161551

   Purpose

   Constitutive expression of intC-ZF1-NLS-HA under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   intC-ZF1-NLS-HA
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJM522 EF1α intC-ZF10-NLS-HA in TUPV2

   Plasmid

   #161552

   Purpose

   Constitutive expression of intC-ZF10-NLS-HA under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   intC-ZF10-NLS-HA
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJB002 CMV 3xFLAG-NLS-DsDed-ZF1

   Plasmid

   #161532

   Purpose

   Constitutive expression ofCMV 3xFLAG-NLS-DsDed-ZF1 under the CMV promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-DsDed-ZF1
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJM503 EF1α 3xFLAG-NLS-ZF1 in TUPV2

   Plasmid

   #161534

   Purpose

   Constitutive expression of 3xFLAG-NLS-ZF1 under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-ZF1
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-synapsin-NLS-mTurquoise2-optoNES-WPRE

   Plasmid

   #159941

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-mTurquoise2-optoNES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-Synapsin-mScarlet-3XNLS-optoNES-WPRE

   Plasmid

   #159948

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mScarlet-3XNLS-optoNES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-Synapsin-YPET-3XNLS-optoNES-WPRE

    Plasmid

    #159947

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    YPET-3XNLS-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-Synapsin-NLS-mTFP1.0-optoNES-WPRE

    Plasmid

    #159945

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTFP1.0-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-Synapsin-NLS-YPET-optoNES-WPRE

    Plasmid

    #159942

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-YPET-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-Synapsin-NLS-mScarlet-optoNES-WPRE

    Plasmid

    #159943

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mScarlet-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-Synapsin-NLS-Dronpa-optoNES-WPRE

    Plasmid

    #159944

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-Dronpa-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-Synapsin-mTurquoise2-3XNLS-optoNES-WPRE

    Plasmid

    #159946

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mTurquoise2-3XNLS-optoNES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE

    Plasmid

    #159975

    Purpose

    optical regulation of nuclear export with shorter relaxation time

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-YPET-optoNES.F434L
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    F434L

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE

    Plasmid

    #159973

    Purpose

    optical regulation of nuclear export with extended relaxation time

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-YPET-optoNES.V416I
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    V416I

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE

    Plasmid

    #159974

    Purpose

    optical regulation of nuclear export with shorter relaxation time

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-YPET-optoNES.I427V
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    I427V

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-HOXD13(-7A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145271

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13(-7A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(-7A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat deleted

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-HOXA13(+7A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145266

    Purpose

    Mammalian Expression of Nuclear Opto HOXA13(+7A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13(+7A)-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHR-HOXA13-IDR-mCherry-CRY2-NLS

    Plasmid

    #145265

    Purpose

    Mammalian Expression of Nuclear Opto HOXA13 IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13wt-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145262

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+8A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRDAX-VALENTIA-016 NLSNoLS-Sirius//BsaI

    Plasmid

    #130197

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-Sirius
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRDAX-VALENTIA-017 NLSNoLS-SuperNova//BsaI

    Plasmid

    #130198

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-SuperNova
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRDAX-VALENTIA-033 NLSNoLS-Eos//BsaI

    Plasmid

    #130214

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-Eos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

    Plasmid

    #133796

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1 (truncated C-terminal domain only)
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    C-terminal domain only

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)

    Plasmid

    #133797

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1(T16A)
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    T16A

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)

    Plasmid

    #133799

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized Leu-AcrIIA1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)

    Plasmid

    #133800

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized Eriv-AcrIIA1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140224

    Purpose

    Constitutive PGK driven expression of intein with miniVPR for 3-way split activator

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    C-intein with miniVPR
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRDAX-VALENTIA-030 NLSNoLS-mVenus//BsaI

    Plasmid

    #130211

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-mVenus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRDAX-VALENTIA-020 NLSNoLS-Neptune//BsaI

    Plasmid

    #130201

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-Neptune
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRDAX-VALENTIA-018 NLSNoLS-Eos//BsaI

    Plasmid

    #130199

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-Eos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid

    #131133

    Purpose

    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

    Depositor

    Ralf Jansen

    Insert

    NLS-HA-2xMCP-EGFP-cMycBirA*
    

    Use

    Lentiviral

    Tags

    NLS, HA, cMyc (N-terminal of BirA*)

    Expression

    Mammalian

    Mutation

    BirA* = BIrA >R118G

    Promoter

    human ubiquitin C (UBC)

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDH-CMV-Tmeff2-3xFlag-EF1-nlsCopGFP

    Plasmid

    #99729

    Purpose

    overexpression of Tmeff2 and CopGFP

    Depositor

    Mengsheng Qiu

    Article

    Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

    Insert

    Tmeff2 (Tmeff2 Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pED55 pRS306 pRPS2-SteND-NLS-mCherry

    Plasmid

    #68277

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7ND NLS mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

    Plasmid

    #68275

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS(4A) mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAVf-EnhCB-lacZnls mir-122 1xBS

    Plasmid

    #35643

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    1 miR-122 target site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CB

    Available since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-28a(+)::NL

    Plasmid

    #141289

    Purpose

    NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1 NL-BCL2L1

    Plasmid

    #113458

    Purpose

    NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    BCL2 like 1 (BCL2L1 Human)
    

    Use

    Luciferase

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28a Gx-NL

    Plasmid

    #169105

    Purpose

    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGEX Smad3 NL

    Plasmid

    #12632

    Depositor

    Rik Derynck

    Article

    Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

    Insert

    Smad3 NL (SMAD3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-L segment of Smad3 (aa 1-211)

    Promoter

    Available since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

43. pcDNA3.1 PA-NL

    Plasmid

    #113445

    Purpose

    ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLenti PA-NL

    Plasmid

    #113453

    Purpose

    Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-NanoLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. ABE-NW-NL

    Plasmid

    #240621

    Purpose

    Adenine base editor with narrowed editing window

    Depositor

    Tingting Jiang

    Insert

    TadA8e(N108Q,M151C,Q154Y)-nCas9 (D10A)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    TadA8e(N108Q,M151C,Q154Y), Cas9(D10A)

    Promoter

    Available since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

    Plasmid

    #60904

    Purpose

    The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

    Plasmid

    #159798

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

    Plasmid

    #122147

    Purpose

    Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Lentiviral

    Tags

    NLS-iLID-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

    Plasmid

    #183775

    Purpose

    AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    NLS-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    CAG

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid

    #102245

    Purpose

    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

    Depositor

    Jacob Corn

    Insert

    dCas9-VPR-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-VPR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge