Addgene

We narrowed to 11,705 results for: cel.2

Showing: 641 - 660 of 11705 results

Results per page:

Sort results by:

pDY2260 hNOLC1 atgRNA Paired Guide 2

Plasmid

#220990

Purpose

hNOLC1 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 atgRNA (NOLC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDY2262 hACTB atgRNA Paired Guide 2

Plasmid

#220992

Purpose

hACTB atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

ACTB atgRNA (ACTB Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-SREBP-2-GFP (A1134-1135)

Plasmid

#204203

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Use

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

Gly to Ala 1134, 1135

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-Teton-puro-shPP1C #2

Plasmid

#198763

Purpose

conditional knockdown of PP1C

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shPP1C #2 (PPP1CC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-attB2-SA(2)-CRY2-mCherry

Plasmid

#160441

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
peSpCas9(1.1)-2×sgRNA (empty, donor)

Plasmid

#80768

Purpose

All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Bcl-2-G128L-A131L-T132L

Plasmid

#220304

Purpose

mammalian expression of Bcl-2-G128L-A131L-T132L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Use

Tags

Emerald GFP

Expression

Mammalian

Mutation

G128L-A131L-T132L

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W

Plasmid

#210613

Purpose

Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Control(2)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX307-SARS-CoV-2-3CL-C145A

Plasmid

#160279

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC-C Sars-CoV-2 N_D3L

Plasmid

#194806

Purpose

Sars-CoV-2 N_D3L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_N_D3L (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

N_D3L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-C Sars-CoV-2 N_D63G

Plasmid

#194807

Purpose

Sars-CoV-2 N_D63G in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_D63G (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

D63G

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-C Sars-CoV-2 N_R203M

Plasmid

#194810

Purpose

Sars-CoV-2 N_R203M in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_R203M (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

R203M

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-C Sars-CoV-2 N_S235F

Plasmid

#194811

Purpose

Sars-CoV-2 N_S235F in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_S235F (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

S235F

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HA-C Sars-CoV-2 N_T205I

Plasmid

#194812

Purpose

Sars-CoV-2 N_T205I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_T205I (N Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

T205I

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-C Sars-CoV-2 NSP12_P314L

Plasmid

#194813

Purpose

Sars-CoV-2 NSP12_P314L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP12_P314L (ORF1ab Sars-CoV-2)

Use

Tags

MAC-C

Expression

Mammalian

Mutation

P314L

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pKLV2-U6gRNA5(ZNF398_5-2)-PGKpuroBFP-W

Plasmid

#211998

Purpose

Express gRNA against ZNF398 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZNF398 (ZNF398 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(AAVS1-2)-PGKpuroBFP-W

Plasmid

#211955

Purpose

Express safe-cutter control gRNA with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

safe-cutter control sgRNA_AAVS1-2 (AAVS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 puro CXCR4 siRNA-2

Plasmid

#12272

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

Plasmid

#169166

Purpose

Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

Insert

3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)

Use

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Use

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge