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Showing: 641 - 660 of 8127 results

  6. mEos3.2trLAT-P

    Plasmid
    #203747
    Purpose
    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLAToC27A (Lat Mouse)
    Use
    Tags
    ER translocation signal - mEos3.2
    Expression
    Mammalian
    Mutation
    C27A
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203747-plasmid-map-sequence-id-403346

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  7. NmMetQ C20A

    Plasmid
    #172112
    Purpose
    Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence
    Depositor
    Douglas Rees
    Article
    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:
    Insert
    Methionine Binding Protein
    Use
    Tags
    10x Histidine Tag and N.meningitidis signal seque…
    Expression
    Bacterial
    Mutation
    Changed Proline 22 to Alanine on MetQ and Cystein…
    Promoter
    T7 Promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172112-plasmid-map-sequence-id-344822

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  8. pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

    Plasmid
    #123354
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    cp-moxCerulean3-moxCerulean3-FLARE-D1ER
    Use
    Tags
    C-terminal ER retention signal (KDEL), cp-moxCeru…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    123354-plasmid-map-sequence-id-311156

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  15. pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

    Plasmid
    #164193
    Purpose
    Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)
    Depositor
    Thomas Oertner
    Article
    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820
    Inserts
    CamPARI2
    Human α Tubulin
    Use
    AAV
    Tags
    6 His tag and NES
    Expression
    Mammalian
    Mutation
    F391W-L398V
    Promoter
    Syn
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    164193-plasmid-map-sequence-id-325364

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  17. pssGFP-hCRT

    Plasmid
    #80022
    Purpose
    Mammalian expression of signal peptide-EGFP-humanCRT
    Depositor
    Chinten James Lim
    Article
    Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi
    Insert
    CRT (CALR Human)
    Use
    Tags
    EGFP and signal peptide
    Expression
    Mammalian
    Mutation
    signal peptide 17aa added before GFP-CRT, will ta…
    Promoter
    CMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 641 - 660 of 8127 results