We narrowed to 13,730 results for: gnal

1. pLenti6_YAP1-V5

   Plasmid

   #173002

   Purpose

   constitutive expression of V5-tagged YAP1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   YAP1-V5 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV7.1_3xFLAG-WWC1

   Plasmid

   #172994

   Purpose

   constitutive expression of FLAG-tagged WWC1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-WWC1 (WWC1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

   Plasmid

   #172104

   Purpose

   Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH6-TEV-spTorA-GFP(C)

   Plasmid

   #169041

   Purpose

   GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

   Depositor

   Siegfried Musser

   Article

   Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

   Insert

   H6-spTorA-GFP
   

   Tags

   6xHis, TEV cleavage site, and spTorA (signal pept…

   Expression

   Bacterial

   Mutation

   The mut3 GFP variant with 5 additional mutations …

   Promoter

   araBAD

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. His10-CD3z-SNAPf-ybbR (XSB507)

   Plasmid

   #78508

   Purpose

   Construct for expressing CD3z-SNAPf-ybbR in E. Coli

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   CD3z (Cytoplasmic domain) (CD247 Human)
   

   Tags

   His10 and SNAPf-ybbR

   Expression

   Bacterial

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB_Cdc42-TomKat-sensor-CAAX

   Plasmid

   #172473

   Purpose

   Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

   Depositor

   Sean R Collins

   Article

   Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

   Insert

   tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
   

   Use

   Piggybac transposon

   Tags

   polybasic and CAAX motif from Kras, tdKatushka2, …

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV7.1_3xFLAG-STK3

   Plasmid

   #172990

   Purpose

   constitutive expression of FLAG-tagged STK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-STK3 (STK3 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcFLAG-CSK-delta SH3 (73 to450 amino acids)

   Plasmid

   #74504

   Purpose

   Overexpression of CSK delta SH3 in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

   Insert

   CSK (CSK Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   del-SH3 (73 to450 amino acids)

   Promoter

   CMV

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

   Plasmid

   #62577

   Purpose

   Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. Flag‐mTim3 (T1 truncation)

    Plasmid

    #49210

    Purpose

    expresses Flag tagged mouse Tim3 (T1 truncation)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 T1 truncation (Havcr2 Mouse)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    T1 truncation ( contains all but the three most C…

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER-GFP Q185N N186C pLVX CMVpuro

    Plasmid

    #246913

    Purpose

    Lentiviral-based expression of EGFP with an ER retention signal sequence and Q185N N186C, an STT3B-specific glcosylation sequon

    Depositor

    William Kaelin

    Article

    Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    ER signal sequence and KDEL ER retention sequence

    Expression

    Mammalian

    Mutation

    Q185N N186C STT3B-specific sequon to allow for fl…

    Promoter

    CMV

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP

    Plasmid

    #178857

    Purpose

    Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670

    Plasmid

    #178858

    Purpose

    Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

    Plasmid

    #62579

    Purpose

    Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

16. p2K7-bsd-UBI-tagRFP-KDEL

    Plasmid

    #114179

    Purpose

    Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Lentiviral

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/Myc-His beta4

    Plasmid

    #16039

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    beta4 (ITGB4 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His10-CD3z-SNAPf-ybbR (XSB507)

    Plasmid

    #78508

    Purpose

    Construct for expressing CD3z-SNAPf-ybbR in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    CD3z (Cytoplasmic domain) (CD247 Human)
    

    Tags

    His10 and SNAPf-ybbR

    Expression

    Bacterial

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcFLAG-CSK-delta SH3 (73 to450 amino acids)

    Plasmid

    #74504

    Purpose

    Overexpression of CSK delta SH3 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

    Insert

    CSK (CSK Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    del-SH3 (73 to450 amino acids)

    Promoter

    CMV

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

    Plasmid

    #62577

    Purpose

    Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only