176,133 results
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pNO286-3
Plasmid#107746PurposeKey genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)DepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialMutationDeleted AA 223 and 512PromoterJ23106 and J23108Available SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV9-X1.1)
Viral Prep#37825-AAV9-X1.1PurposeReady-to-use AAV9-X1.1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the AAV9-X1.1 serotype, which permits efficient transduction of brain endothelial cells. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BGLF4
Plasmid#195811PurposeGateway Entry Clone of Epstein-Barr virus BGLF4DepositorInsertBGLF4 (BGLF4 Epstein-Barr virus)
UseGateway CloningAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIN BRCA2 WT
Plasmid#16245DepositorInsertBRCA2 (BRCA2 Human)
ExpressionMammalianAvailable SinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_r5-HT1.0
Plasmid#208713PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cellsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
ExpressionMammalianPromoterCMVAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-CANTHipi
Plasmid#53301PurposeContains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.DepositorInsertcrtO
UseLow copy number bacterial cloning vectorTags6HisPromoterTrcAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
ampkar
Plasmid#35097PurposeA fluorescent reporter of AMPK activity and cellular energy stressDepositorInsertampkar
TagsECFP and VenusExpressionBacterialMutationnaPromoterCMVAvailable SinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4591NES
Plasmid#138377PurposeBooster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.DepositorInsertBooster-ROCK
ExpressionMammalianPromoterCAGAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flpe
Plasmid#13787PurposeMammalian expression of enhanced form of Flp recombinase with C-terminal Myc tagDepositorInsertFlpe
TagsMycExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-AtpU6-26
Plasmid#160218PurposeAtpU6-26 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-26 promoter
UseGolden gate level 0 piece to express grnasAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_DENV3_PrME
Plasmid#207265PurposeMammalian expression of Dengue virus 3 PrME proteinDepositorInsertDENV3_PrME (POLY Dengue virus 3)
ExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BALF5
Plasmid#198577PurposeGateway Entry CloneDepositorAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUK21
Plasmid#49788PurposeE. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)DepositorTypeEmpty backboneUseCloning vectorPromoterlacZAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFPC1-hMyoX
Plasmid#47608DepositorInsertMyoX (MYO10 Human)
TagsEGFP-HA-HisExpressionMammalianMutationSee depositor comments below.PromoterCMVAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-BFP
Plasmid#188776PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionPromoterSFFVAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_ARP2
Plasmid#111658PurposeMAC-tagged gene expressionDepositorInsertARP2 (ACTR2 Human)
ExpressionMammalianAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV2)
Viral Prep#105547-AAV2PurposeReady-to-use AAV2 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEGFPAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
SRSF8_RRM_pGEX
Plasmid#135119PurposeRecombinant expression of SRSF8 with dual-affinity tagDepositorAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXA1
Plasmid#153109PurposeExpression of Flag tagged human FOXA1 in mammalian cellsDepositorAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRPM2_pCSdest
Plasmid#53920DepositorAvailable SinceSept. 8, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
P362-FBP_12
Plasmid#139703PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Dcp1a
Plasmid#153972PurposeFluorescent protein fused to mRNA decapping enzyme 1aDepositorAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_L_MARV
Plasmid#135473PurposeMammalian expression vector encoding Marburg virus L gene under control of the AG promoterDepositorInsertL gene
ExpressionMammalianMutationsilent mutation (C to T) at position 7861; insert…PromoterAGAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_Cas9
Plasmid#126029PurposePiggyBac cargo vector expressing doxycycline inducible Cas9DepositorInsertshumanized S. pyrogenes Cas9
Hygromycin resistance
UseCRISPR; PiggybacExpressionMammalianPromoterEF1 alpha and TREAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG GST DEPDC5
Plasmid#164983PurposeDEPDC5 expressing vector for mammalian cellsDepositorAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterMyl7 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
B. subtilis Single Gene Deletion Library--Kanamycin
Plasmid Kit#1000000115PurposeA collection of Bacillus subtilis strains in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.DepositorApplicationStrain CollectionsVector TypeBacterial ExpressionAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)
Plasmid#208942PurposeThe CE1 construct, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BXLF1
Plasmid#195825PurposeGateway Entry Clone of Epstein-Barr virus BXLF1DepositorInsertBXLF1 (BXLF1 Epstein-Barr virus)
UseGateway CloningAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only