161,377 results

1. mDsRed-C1

   Plasmid

   #54502

   Purpose

   Localization: C1 Cloning Vector, Excitation: 557, Emission: 585

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mDsRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

2. p15aC-1D

   Plasmid

   #107863

   Purpose

   Bacterial expression of PIS

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Insert

   PIS
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   proD

   Available since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TERT - R865C

   Plasmid

   #213926

   Purpose

   Expresses Mutant TERT R865C

   Depositor

   Coleman Lindsley

   Article

   Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

   Insert

   Mutant TERT R865C (TERT Human)
   

   Use

   Lentiviral

   Tags

   6x His Tag

   Expression

   Mammalian

   Mutation

   Arginine 865 changed to Cysteine

   Promoter

   Tet-responsive promoter PTight, consisting of sev…

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLVX-PERK(LD)-EGFP

   Plasmid

   #225680

   Purpose

   Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

   Depositor

   Likun Wang

   Article

   Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO.1-puro U6 sgRNA BfuAI large stuffer

   Plasmid

   #52628

   Purpose

   lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency

   Depositor

   Scot Wolfe

   Insert

   Kanamycin cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDY0663 ACTB atgRNA with v2 scaffold

   Plasmid

   #179108

   Purpose

   ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CL20_mEGFP_EML4_ALK

   Plasmid

   #205809

   Purpose

   Express mEGFP-tagged fusion protein, EML4_ALK from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   EML4_ALK (EML4, ALK Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE-ERBB2

   Plasmid

   #116734

   Purpose

   Lentiviral expression of ERBB2

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSH124-ssr

   Plasmid

   #198025

   Purpose

   Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   T0 terminator - Ap/Cb resistance cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ApR promoter

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET His6 MBP Asn10 TEV LIC cloning vector (1C)

    Plasmid

    #29654

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

    Plasmid

    #205443

    Purpose

    human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD46-HLA-G SCT-hCD47-hCD59
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. loSIIN

    Plasmid

    #185663

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAS162

    Plasmid

    #194485

    Purpose

    synGR Cassette

    Depositor

    Ahmad Khalil

    Article

    Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

    Insert

    synGR Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSico

    Plasmid

    #11578

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. eGFP STAT1 S727E

    Plasmid

    #12305

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Jun 26. ():.

    Insert

    STAT1 S727E (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S727E

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag

    Plasmid

    #231591

    Purpose

    Express circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-2xALFATag-10xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-GFP11x7-HygR

    Plasmid

    #223676

    Purpose

    To check the split GFP signals in mitochondria (2)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP11x7
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti PGK Hygro DEST (w530-1)

    Plasmid

    #19066

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-N3 alpha-SSeCKS

    Plasmid

    #61294

    Purpose

    Mammalian expression of GFP-alpha-SSeCKS

    Depositor

    Irwin Gelman

    Insert

    alpha SSeCKS (Akap12 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pET-Duet1-His-TEV-mCherry-OPTN WT

    Plasmid

    #190191

    Purpose

    Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    6xHis, TEV, and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLVX-NPM1c-IRES-mCherry

    Plasmid

    #131825

    Purpose

    Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. UKNP1.21.5

    Plasmid

    #98203

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

    Plasmid

    #199371

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti6.2_mCherry_roGFP2_Orp1

    Plasmid

    #155043

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE2-TetOminiCMV-OSK

    Plasmid

    #136552

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CaVα2δ1

    Plasmid

    #26575

    Depositor

    Diane Lipscombe

    Article

    Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

    Insert

    Cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pExp-MBP

    Plasmid

    #112568

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-MBP and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

    Plasmid

    #193942

    Purpose

    a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

    Depositor

    Greg Delgoffe

    Article

    Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

    Insert

    heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEM mouse islet1 [TJ#28]

    Plasmid

    #16270

    Depositor

    Thomas Jessell

    Insert

    islet1 (Isl1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only