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mActA-mSspB-mCherry

Plasmid

#210294

Purpose

ActuAtor for iLID optogenetics

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

mActA-,SspB-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mBeRFP

Plasmid

#228620

Purpose

Mammalian expression of mBeRFP and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mBeRFP

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mTFP1

Plasmid

#228621

Purpose

Mammalian expression of mTFP1 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mTFP1

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-CyOFP1

Plasmid

#228623

Purpose

Mammalian expression of CyOFP1 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

CyOFP1

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mOrange2

Plasmid

#228627

Purpose

Mammalian expression of mOrange2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mOrange2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mRuby3

Plasmid

#228628

Purpose

Mammalian expression of mRuby3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mRuby3

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-Cdc42-T17N

Plasmid

#214289

Purpose

Express dominant negative Cdc42 fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Dominant negative Cdc42 (CDC42 Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Cdc42-T17N

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-Rac1-Q61L

Plasmid

#214292

Purpose

Express constitutively active Rac1 fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Constitutively active Rac1 (RAC1 Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Rac1-Q61L

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-Rac1-T17N

Plasmid

#214293

Purpose

Express dominant negative Rac1 fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Dominant negative Rac1 (RAC1 Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Rac1-T17N

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-RhoA-Q63L

Plasmid

#214296

Purpose

Express constitutively active RhoA fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Constitutively active RhoA (RHOA Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

RhoA-Q63L

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-RhoA-T19N

Plasmid

#214297

Purpose

Express dominant negative RhoA fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Dominant negative RhoA (RHOA Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

RhoA-T19N

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Plasmid

#216403

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 70, 443 to P…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBCC086

Plasmid

#202213

Purpose

Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

pBCC25, pBCC33, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBCC091

Plasmid

#202218

Purpose

Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

pBCC25, pBCC30, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBCC100

Plasmid

#202226

Purpose

Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

pBCC25, pBCC32, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-myr-rsEGFP2- LDLR

Plasmid

#213399

Purpose

double floxed, myristoylation site (myr) and LDLR, fused to reversibly switchable EGFP rsEGFP2

Depositor

Katrin Willig

Article

Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

Insert

rsEGFP2

Use

AAV

Tags

C-terminal (Ct) cytoplasmic domains of low densit…

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28 sf:em-blue-shift.1

Plasmid

#191928

Purpose

Encodes a GFP design, sf:em-blue-shift.1

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:em-blue-shift.1

Tags

His Tag

Expression

Bacterial

Mutation

T65S Q69P S72A T108V Y145M T167V V224I

Promoter

T7

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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sgVRK2-puro

Plasmid

#199636

Purpose

sgRNA guide against VRK2

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK2 Human)

Use

Lentiviral

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPIP1;4-NLS-3xmCitrine

Plasmid

#192524

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

PIP1;4

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK2-K168E

Plasmid

#199642

Purpose

Expresses kinase-inactive VRK2 (K168E)

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK2 (VRK2 Human)

Use

Lentiviral

Mutation

K168E

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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