161,377 results
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HA-EGFP-HaloTag2
Plasmid#41742DepositorInsertHalotag 2
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCYP64
Plasmid#212897PurposeExpresses the Priestia megaterium LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationP450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…PromoterPcymRCAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFTK055
Plasmid#171327PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertrTA2S-M2 (TetR-3xVP16) transcriptional activator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-nCas9-UdgX
Plasmid#163566PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-nCas9-UdgX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV9)
Viral Prep#26975-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-DPP4-puro
Plasmid#158451PurposeConstitutive lentiviral expression of human DPP4.DepositorInsertDPP4 (DPP4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO deleted ITR
Plasmid#67906PurposePiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeatDepositorInsertNeoR (neoR Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover
Plasmid#163366PurposeBackbone for generating N-terminal or C-terminal clover constructsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-OSF-PP
Plasmid#154247Purposebackbone with PreScission protease-cleavable N-terminal OneStrepFLAG tagDepositorTypeEmpty backboneUseTagsFLAG and Strep-Tag IIExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
mAIRN HO
Plasmid#207411PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAIRN CD
Plasmid#207412PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SCN1B-Fc(DAPA)-AviTag-6xHis
Plasmid#156534PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSCN1B (SCN1B Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-PKCgamma-C1A
Plasmid#21205DepositorInsertPKCgamma-C1A (Prkcg Rat)
UseTagsGFPExpressionMammalianMutationPKCgamma-C1A domain. (Lab plasmid WC 0018)PromoterAvailable sinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-GBP
Plasmid#67650PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertAPEX2-GBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eCas9-GFP-U6-gRNA
Plasmid#79145PurposeAll-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cellsDepositorInserteSpCas9(1.1)
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GFP-CAAX
Plasmid#184813PurposeUsed to generate the Tg(QUAS:GFP-CAAX)c591 transgenic lineDepositorInsertQUAS:GFP-CAAX-pA
UseZebrafish tol2 transgenesisTagsGFP-CAAXExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-GEM-GECO1
Plasmid#32461PurposeMammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorInsertGEM-GECO
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHN1002
Plasmid#178831PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, 3CExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORF1-2
Plasmid#206024PurposeAn expression vector for mouse LINE1 ORF1-2DepositorInsertORF1-2 derived from mouse LINE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM464 (deletions)
Plasmid#108322PurposeOrbit integrating plasmid to delete target gene.DepositorInsertBxb1 attB site
UseOrbit integration plasmid for creating knockouts …TagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pD649-HAsp-TREM2-COMP5AP-AviTag-9xHis
Plasmid#157115PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTREM2 (TREM2 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
mu-GM-CSF-pCIneo
Plasmid#163518PurposeExpresses murine GM-CSF in mammalian cellsDepositorInsertmurine GM-CSF
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet-I
Plasmid#104007PurposePlasmid encoding mScarlet-IDepositorInsertmScarlet-I
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.23.1
Plasmid#98206PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
phOCT4-Luc
Plasmid#17221DepositorInsertUpstream region of human OCT4 (POU5F1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Nesprin3-C-18
Plasmid#54203PurposeLocalization: Nuclear Membrane , Excitation: 487, Emission: 509DepositorInsertNesprin3 (SYNE3 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits
