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pEPOR2KN0038

Plasmid

#117605

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pUAP1_Ta_Yr7_CDS_no_STOP

Plasmid

#141093

Purpose

Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

Depositor

Cristobal Uauy

Article

Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

Insert

Ta_Yr7

Use

Unspecified; Level 0 module for golden gate cloni…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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JH05

Plasmid

#73516

Purpose

mYPet_PH pCFJ150 - contain C. elegans codon optimized mYPet

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mPYet::PH::tbb-2

Tags

mYPet

Promoter

mex-5

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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HL 3904 (=HL 770 + pHL 991)

Plasmid

#30093

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
Dom34+GST

Plasmid

#19099

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag, GST, and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
psiCHECK SIRT4 3'UTR

Plasmid

#19860

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

SIRT4 3' UTR (SIRT4 Human)

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG1

Plasmid

#166846

Purpose

Expresses Atg1 with ATG1 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Promoter

ATG1 Promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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Ta1098

Plasmid

#19072

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pSR305

Plasmid

#124712

Purpose

Expression of uhpB under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpB (uhpB )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40

Plasmid

#124604

Purpose

Cre-dependent soma-localized C1V1(t/t) for optogenetic stimulation

Depositor

Christopher Harvey

Article

Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

Insert

Soma-localized C1V1(t/t)

Use

AAV

Tags

mRuby2

Expression

Mammalian

Promoter

Ef1a

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-At 14-3-3 lambda

Plasmid

#44942

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 lambda (GRF6 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
px330BRG1 gRNA

Plasmid

#165585

Purpose

Insertion of BRG1 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

BRG1 (Smarca4 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox c-SPT5_EYFP_FKBPF36V_Neo

Plasmid

#163872

Purpose

Acute Depletion of SPT5-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Spt5 (Supt5 Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox_N-BRG1_BSD_FKBPF36V_EYFP

Plasmid

#163874

Purpose

Acute Depletion of BRG1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brg1 (Smarca4 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A IRES GFP

Plasmid

#133818

Purpose

Encodes the C2A domain of synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SERA6-bio-His

Plasmid

#50818

Purpose

Expresses enzymatically monobiotinylated full length SERA6

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA6

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-p65(150-261)-RTA(125-190)

Plasmid

#99674

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

VP64-P65(151-261)-RTA(125-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCFD3-MS2tail-Scaffold

Plasmid

#78906

Purpose

Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP (F64L, S65T, V163A)-kanMX6

Plasmid

#53183

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

kanMX6

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsXRN1_1650-1706_P

Plasmid

#147250

Purpose

Mammalian Expression of HsXRN1_1650-1706

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

HsXRN1_1650-1706 (XRN1 Human)

Expression

Mammalian

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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