We narrowed to 66,569 results for: des.1
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Plasmid#81053Purposebacterial expression of the catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
Tags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057PromoterT7Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
WN10151
Plasmid#80441PurposeExpresses human codon-optimized dead (832A) LbCpf1DepositorInsertdead (D832A) LbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832AAvailable SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJR83
Plasmid#75028PurposemCherry^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmCherry^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized mCherryExpressionWormAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTiGc
Plasmid#78314PurposeMycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).DepositorInsertsTurboFP635
GFP
UseSynthetic Biology; Mycobacterial replicating vect…ExpressionBacterialPromoterhsp60 and hsp60(ribo)Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Synapsin sHRPb-NLG in FSW lentiviral vector
Plasmid#73148PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.DepositorInsertsHRPb-NLG (Nlgn1 Synthetic, Mouse)
UseLentiviralTagsV5 tagExpressionMammalianPromoterSynapsinAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_Lifeact-GFP_NLS-mCherry
Plasmid#69058PurposeDual Fluorescent Reporter for F-actin and NucleiDepositorInsertLifeAct-GFP_IRES_NLS-mCherry
TagsLifeact-GFP, NLS-mCherryExpressionMammalianPromoterCMVAvailable SinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FOXA1_#2
Plasmid#70096PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT-Bip-GFP:V5:KDEL
Plasmid#69917PurposeAn endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.DepositorInsertGFP
TagsDrosophila BiP signal sequence, KDEL ER targeting…ExpressionInsectPromoterpMTAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9/pTREX-n
Plasmid#68708PurposeExpresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.DepositorInsertFusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
UseCRISPRTagsFusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …MutationC4239T mutation that eliminates a BamHI restricti…Available SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-emerin-EGFP-polyA
Plasmid#59747PurposeExpress emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertemerin-EGFP
TagsEGFPExpressionMammalianPromoterCMV and T7Available SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-flex-oChIEF-citrine
Plasmid#50973Purposeflexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITRDepositorInsertChIEF-citrine
UseAAVTagscitrineMutationinverted and inserted between two lox sitesPromoterhuman synapsin promoterAvailable SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PML-3/pSG5
Plasmid#50939Purposeexpression of PML with Sumo sites destroyedDepositorInsertPML-3 (PML Human)
TagsHAExpressionMammalianMutationK65R, K160R, K490R (Sumo modification sites)PromoterSV40Available SinceJan. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgG4/λ
Plasmid#50369PurposeExpresses grass pollen allergen Phl p 7 specific human IgG4/λ antibody isotype (102.1F10-IgG4/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 4 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
ExpressionMammalianPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA.Tetherin
Plasmid#41068DepositorAvailable SinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1.ARRDC1
Plasmid#38320DepositorAvailable SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc human ERBIN
Plasmid#40214DepositorInsertERBIN (ERBIN Human)
TagsmycExpressionMammalianMutationL143S, S274L and P338L mutation in ERBIN compared…PromoterCMVAvailable SinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
HA-V2R
Plasmid#37061DepositorAvailable SinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEBB HA cIAP1
Plasmid#38232DepositorAvailable SinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-hChR2_tdTomato
Plasmid#28015PurposeExpresses humanized ChR2 fused to tdTomato for optogenetic activationDepositorInserthumanized ChR2-tdTomato
UseAAV; Adeno associate viralTagstdTomatoPromoterCAGAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,42a] mut
Plasmid#26576DepositorInsertCACNA1D (Cacna1d Rat)
Tags6XHis and V5ExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…Available SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only