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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 92,501 results for: lias

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Showing: 10141 - 10170 of 92501 results

  10. cycLuc_TEVS

    Plasmid
    #119207
    Purpose
    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with TEVs cleavage site
    Use
    Luciferase
    Tags
    6xHis and AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119207-plasmid-map-sequence-id-232048

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  18. CT691

    Plasmid
    #233660
    Purpose
    BLADE (two-input, four-output decoder); Cre excises lox sites to express GFP, Flp excises FRT sites to express iRFP, and the presence of both recombinases is designed to express mRuby.
    Depositor
    Wilson Wong
    Article
    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi
    Insert
    BLADE
    Expression
    Mammalian
    Mutation
    None
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    233660-plasmid-map-sequence-id-464957

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  20. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

    Plasmid
    #169550
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Expression
    Mammalian
    Promoter
    PPGK
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169550-plasmid-map-sequence-id-366597

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  22. pDV06.2c_DART-VADAR_iRFP720-sensor

    Plasmid
    #200410
    Purpose
    pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    Closed-loop DART VADAR sensor detecting iRFP720
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200410-plasmid-map-sequence-id-396811

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  24. 3xFLAG-dCas9/pMXs-neo

    Plasmid
    #51260
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    51260-plasmid-map-sequence-id-77871

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  29. FpG5

    Plasmid
    #69443
    Purpose
    lentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAs
    Depositor
    Lisa Dailey
    Article
    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:
    Inserts
    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    69443-plasmid-map-sequence-id-122529

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Showing: 10141 - 10170 of 92501 results