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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,992 results for: Met

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Showing: 661 - 680 of 18992 results

  9. pALS3-Ma-sfGFP-TAG150

    Plasmid
    #212121
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212121-plasmid-map-sequence-id-444186

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  10. pBK-Ma pylRS nitroY/haloY-F5

    Plasmid
    #212125
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.
    Depositor
    Ryan Mehl
    Insert
    M. alvus PylRS 3-NY/HaloY F5
    Expression
    Bacterial
    Mutation
    CTG125CTT, N166A V168S, A223C, W239R
    Promoter
    GlnS (constitutive)
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    212125-plasmid-map-sequence-id-443626

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  13. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

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  19. pCDF-5xT7-TtCsm

    Plasmid
    #128572
    Purpose
    For expression of T. thermophilus Csm in E. coli (Csm1-5)
    Depositor
    Jennifer Doudna
    Article
    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:
    Inserts
    Csm1
    Csm2, Csm3, Csm4, Csm5
    Tags
    HRV 3C protease cleavage site and 10xHis tag on C…
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli and Cod…
    Promoter
    T7 promoter, lac operator
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128572-plasmid-map-sequence-id-252577

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Showing: 661 - 680 of 18992 results