We narrowed to 28,294 results for: cmv promoter

Showing: 10201 - 10230 of 28294 results

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pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2_C277S-Flag

Plasmid

#180412

Purpose

lentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tag

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2_C277S (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C277S

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Venus/Cerulean-Synaptopodin-A in pcDNA6

Plasmid

#190798

Purpose

Imaging of synaptopodin in live cells

Depositor

Vivian Tang

Article

Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

Insert

Synaptopodin-A (SYNPO Human, Synthetic)

Tags

Cerulean, His, Venus, and myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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HERV-Kcon-RT for Split-PE (pJUL3120)

Plasmid

#190107

Purpose

Expresses HERV-Kcon RT (human codon optimization)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

HERV-Kcon-RT

Tags

bpNLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

Plasmid

#187576

Purpose

Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

LMNA (LMNA Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

Plasmid

#187574

Purpose

Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

RNPS1 (RNPS1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat-mCherry

Plasmid

#181841

Purpose

mCherry-tagged PKA catalytic subunit for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR

Plasmid

#181830

Purpose

Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-ExRai-AktAR2

Plasmid

#184048

Purpose

Cytosol-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Cyto-ExRai-AktAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AktAR2

Plasmid

#184049

Purpose

Nuclear-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Nuc-ExRai-AktAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSN837

Plasmid

#184825

Purpose

Expresses human KIF5A in mammalian cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (KIF5A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 WC visual barcode

Plasmid

#158666

Purpose

tagging clonal populations with whole cell mTAGBFP2 fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

Plasmid

#182925

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Tags

APEX2 and FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304-DCK*-IRES-Td

Plasmid

#176298

Purpose

Expresses DCK* and TdTomato from the same transcript

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-mKOR

Plasmid

#179285

Purpose

Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

Depositor

Christian Gruber

Article

Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

Insert

kappa opioid receptor (Oprk1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-SorLA-v2

Plasmid

#154900

Purpose

Expresses SorLA fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

SORL1 (SORL1 Human)

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_WT-talin1 (1-2541)

Plasmid

#166112

Purpose

For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

WT-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEXPqcxip-hSURF4-FLAG

Plasmid

#159139

Purpose

Hu SURF4 -flag mammalian expression Gateway vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

surfeit 4 (SURF4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV (cytomegalovirus) enhancer and the MSV (mouse…

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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GZnP2 in pcDNA3.1+

Plasmid

#161739

Purpose

Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

Depositor

Yan Qin

Article

Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

Insert

GZnP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term His8 GFP

Plasmid

#160680

Purpose

vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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IMS-HyPer2

Plasmid

#60248

Purpose

Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

IMS-HyPer2

Tags

Mitochondrial intermembrane space targeting signa…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16

Plasmid

#158742

Purpose

Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Neuraminidase A/NL/354/16 H3N2

Tags

TwinStrep_sfGFP_tetrabrachion

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EphrinB3-Flag

Plasmid

#155012

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

EphrinB3 (EPHB3 Human)

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_CEACAM6-His

Plasmid

#149337

Purpose

for eukaryotic expression and purification of CEACAM6-His6

Depositor

James Fleckenstein

Article

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Tags

IL-2 signal peptide and polyhistidine

Expression

Mammalian

Mutation

point mutation (GGA to GCA) to replace 'G-g…

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

Plasmid

#159455

Purpose

Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

Depositor

Oliver Griesbeck

Article

Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

Insert

mCRISPRed

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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DD-DamWT-IRES2-mCherry

Plasmid

#159602

Purpose

Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the wild-type allele of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamWT-IRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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DD-DamWT-LMNB1-IRES2-mCherry

Plasmid

#159601

Purpose

Shield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the wild-type allele of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamWT-LMNB1-IRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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DD-DamV133A-LMNB1-IRES2-mCherry

Plasmid

#159599

Purpose

Shield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the V133A mutant of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamV133A-LMNB1-IRES2-mCherry

Expression

Mammalian

Mutation

Dam V133A mutation

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR-WT

Plasmid

#159159

Purpose

Expresses DEPTOR in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXQ1

Plasmid

#153149

Purpose

Expression of Flag tagged human FOXQ1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXQ1 (FOXQ1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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