We narrowed to 37,209 results for: ELL

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pBabe puro RalA (long) wt

Plasmid

#19717

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

NOTE: long splice variant with 3bp deletion and 1…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pEXL flag Smad3

Plasmid

#10920

Depositor

Bob Weinberg

Article

Liu et al Proc Natl Acad Sci U S A. 1997 Sep 30. 9

Insert

Smad3 (SMAD3 Human)

Tags

flag

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCAGGS-PHFF-sh-mBVRA

Plasmid

#100286

Purpose

Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Inserts

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for mouse BVRA

Tags

FLAG, T7 and Mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

All genes are codon-optimized for expression in h…

Promoter

CAG promoter and H1 promoter

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBait-YY1

Plasmid

#165147

Purpose

Bait plasmid for PROBER with YY1-binding site (positive control)

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

3X YY1 motif

Use

Unspecified

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSprayer

Plasmid

#165148

Purpose

Sprayer plasmid for PROBER encoding BASU-GAL4 fusion

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

BASU-GAL4

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMH0004

Plasmid

#174087

Purpose

UCOE-SFFV-Cas9-BFP

Depositor

Jonathan Weissman

Article

Hein et al Nat Biotechnol. 2022 Mar;40(3):391-401.

Insert

Cas9-BFP

Use

Lentiviral

Promoter

SFFV

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

Plasmid

#227246

Purpose

All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

Depositor

Jae Seong Lee

Article

Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

Inserts

Tet-On 3G transactivator protein
Enhanced green fluorescent protein

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

EF1α and pTRE3G

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

Plasmid

#182925

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Tags

APEX2 and FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD9-pHuji

Plasmid

#130906

Purpose

Expression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD9-pHuji (CD9 Synthetic, Human)

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Asn50 and Asn51 in extrace…

Promoter

CMV

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCG004

Plasmid

#87377

Purpose

ORF part assembly entry vector derived from pHT01.

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

GFP dropout part

Expression

Bacterial

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pZS2oxySp-GFP-proD-oxyR

Plasmid

#78224

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEN-MCS

Plasmid

#44919

Depositor

Harry Mobley

Article

Lane et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Stable complementation

Promoter

none

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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p4B5-EGFP

Plasmid

#140324

Purpose

Detection of Dengue virus infection in live cells

Depositor

Alan Rothman

Article

Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

Insert

DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP

Tags

eGFP

Expression

Mammalian

Mutation

2 conservative n.t. differences near 5' end …

Promoter

CMV

Available Since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

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rescue plasmid

Plasmid

#135971

Purpose

expresses copGFP and puromycin resistance genes

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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VPR_dCas9

Plasmid

#134601

Purpose

Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

Depositor

Lasse Ebdrup Pedersen

Article

Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

Insert

VPR-dCas9-2A-ZsGreen-DR

Tags

VPR and ZsGreen1-DR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-KIRIN1-GR

Plasmid

#107417

Purpose

Bacterial Expression plasmid for potassium ion ratiometric indicator KIRIN1-GR

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1-GR

Expression

Bacterial

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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MCS Lck only

Plasmid

#90411

Purpose

A control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.

Depositor

Katharina Gaus

Article

Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

Insert

Lck10+ Venus+mcherry

Expression

Mammalian

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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RFP-(SUMO)10-(SIM)6

Plasmid

#122026

Purpose

Mammalian expression plasmid for multivalent scaffold of (SUMO)10-(SIM)6 biomolecular condensates to recruit SIM-containing client proteins.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 copies of human SUMO3 and 6 repeats of human SUMO interactive motif (SIM) from PIASx separated by (GGS)4 linker (SUMO3, PIAS2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo

Plasmid

#78353

Purpose

Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag HCK

Plasmid

#20497

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HCK (HCK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only